Zinc ions prevent α-synuclein aggregation by enhancing chaperone function of human serum albumin.

Samah Al-Harthi, Vladlena Kharchenko, Papita Mandal, Spyridon Gourdoupis, Lukasz Jaremko

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Metal ions present in cellular microenvironment have been implicated as drivers of aggregation of amyloid forming proteins. Zinc (Zn2+) ions have been reported to directly interact with α-synuclein (AS), a causative agent of Parkinson's disease and other neurodegenerative diseases, and promote its aggregation. AS is a small intrinsically disordered protein (IDP) i.e., understanding molecular factors that drive its misfolding and aggregation has been challenging since methods used routinely to study protein structure are not effective for IDPs. Here, we report the atomic details of Zn2+ binding to AS at physiologically relevant conditions using proton-less NMR techniques that can be applied to highly dynamic systems like IDPs. We also examined how human serum albumin (HSA), the most abundant protein in human blood, binds to AS and whether Zn2+ and/or ionic strength affect this. We conclude that Zn2+ enhances the anti-aggregation chaperoning role of HSA that relies on protecting the hydrophobic N-terminal and NAC regions of AS, rather than polar negatively charged C-terminus. This suggested a previously undocumented role of Zn2+ in HSA function and AS aggregation.
Original languageEnglish (US)
JournalInternational Journal of Biological Macromolecules
StatePublished - Oct 14 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-10-20
Acknowledged KAUST grant number(s): OSR-CRG2018-3792, OSR-CRG2019-4088
Acknowledgements: This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-CRG2018-3792 and Award No. OSR-CRG2019-4088 (L.J.).

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Molecular Biology


Dive into the research topics of 'Zinc ions prevent α-synuclein aggregation by enhancing chaperone function of human serum albumin.'. Together they form a unique fingerprint.

Cite this