Zaxinone Synthase controls arbuscular mycorrhizal colonization level in rice

Cristina Votta, Valentina Fiorilli, Imran Haider, Jian You Wang, Raffaella Balestrini, Ivan Petřík, Danuše Tarkowská, Ondrej Novak, Akmaral Serikbayeva, Paola Bonfante, Salim Al-Babili, Luisa Lanfranco

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The rice carotenoid cleavage dioxygenase OsZAS was described to produce zaxinone, a novel plant-growth promoting apocarotenoid. A zas mutant line showed a reduced arbuscular mycorrhizal (AM) colonization but the mechanisms underlying this behavior are unknown. Here, we investigated how OsZAS and exogenous zaxinone treatment regulate mycorrhization. Micromolar exogenous supply of zaxinone rescued the root growth but not the mycorrhizal defects of the zas mutant and even reduced mycorrhization in wild type and zas genotypes. The zas line did not display an increase in strigolactones (SLs) level as observed in wild type plants at 7 day post inoculation with the AM fungus. Moreover, an exogenous treatment with the synthetic SLs analog, GR24, rescued the zas mutant mycorrhizal phenotype, indicating that the lower AM colonization rate of zas is due to a SLs deficiency at the early stages of the interaction and pointing out that, during this phase, OsZAS activity is required to induce SLs production, possibly mediated by the Dwarf14-Like (D14L) signaling pathway. OsZAS is expressed in arbuscule-containing cells and OsPT11prom
Original languageEnglish (US)
JournalThe Plant Journal
DOIs
StatePublished - Jul 25 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-09-14
Acknowledged KAUST grant number(s): CRG2017
Acknowledgements: This work was supported by baseline funding of the University of Turin (LL and VF) and the Competitive Research Grant (CRG2017) given to SA-B and LL from King Abdullah University of Science and Technology and by the Ministry of Education, Youth and Sports of the Czech Republic (European Regional Development Fund-Project “Centre for Experimental Plant Biology” No. CZ.02.1.01/0.0/0.0/16_019/0000738), and the Internal Grant Agency of Palacký University (IGA_PrF_2021_011) to ON. The authors thank Dr. Mara Novero and Dr. Mara Politi for their technical assistance and Prof. Andrea Genre for providing chito-olisaccharides.

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

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