Xeno-free self-assembling peptide scaffolds for building 3D organotypic skin cultures

Yihua Loo, Andrew C.A. Wan, Charlotte Hauser, E. Birgitte Lane, Paula Benny

Research output: Contribution to journalArticlepeer-review

Abstract

Organotypic skin cultures represent in vitro models of skin which can be used for disease modeling, tissue engineering, and screening applications. Non-human collagen is currently the gold standard material used for the construction of the supporting matrix, however, its clinical applications are limited due to its xenogeneic origin. We have developed a novel peptide hydrogel-based skin construct that shows a pluristratified epidermis, basement membrane, and dermal compartment after 3 weeks of in vitro culture. Peptide-based constructs were compared to collagen-based constructs and stratification marker expression was histologically higher in peptide constructs than in collagen constructs. Transepithelial electrical resistance also showed mature barrier function in peptide constructs. This study presents a novel application of the self-assembling peptide hydrogel in a defined xeno-free in vitro system.
Original languageEnglish (US)
JournalFASEB BioAdvances
DOIs
StatePublished - Jun 11 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-07-01
Acknowledgements: This work was supported by the Institute of Bioengineering and Nanotechnology, Institute of Medical Biology, and Biomedical Research Council (Agency for Science, Technology and Research, Singapore). Yihua Loo was also supported by the A*STAR BMRC YIG Grant 14/1/07/51/011.

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