Wheat doubled haploids have a marked prevalence of chromosomal aberrations

Sandesh Shrestha; Dal-Hoe Koo; Byron Evers; Shuangye Wu; Sean Walkowiak; Pierre Hucl; Curtis Pozniak; Allan K. Fritz; Jesse Poland

doi:10.1002/tpg2.20309

Wheat doubled haploids have a marked prevalence of chromosomal aberrations

Sandesh Shrestha, Dal-Hoe Koo, Byron Evers, Shuangye Wu, Sean Walkowiak, Pierre Hucl, Curtis Pozniak, Allan K. Fritz, Jesse Poland

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Double haploid (DH) population development is widely used in many crops, including wheat (Triticum aestivum L.), to rapidly produce fixed germplasm for breeding and genetic studies. The genome shock that takes place during DH induction could induce chromosomal aberrations that can impact genome integrity and subsequently plant fitness and agronomic performance. To evaluate the extent of chromosomal aberrations that exist as a result of the DH process, we studied two wheat DH populations: CDC Stanley×CDC Landmark and KS13H9×SYMonument. We utilized high-throughput skim sequencing to construct digital karyotypes of these populations to quantify deletions and aneuploidy with high resolution and accuracy, which was confirmed in selected plants by cytological analysis. The two populations studied showed high proportion of abnormal primary DH lines, 55 and 45%, respectively, based on at least one abnormality per progeny. The chromosomal abnormalities are genetically unstable and were observed segregating in the subsequent generations. These observations have important implications for the use of DH lines in genetics and breeding.

Original language	English (US)
Journal	The Plant Genome
DOIs	https://doi.org/10.1002/tpg2.20309
State	Published - May 1 2023

Bibliographical note

KAUST Repository Item: Exported on 2023-05-05
Acknowledged KAUST grant number(s): BAS/1/1098-01-01
Acknowledgements: This project was completed with support from the Kansas Wheat Commission and the Kansas Wheat Alliance, King Abdullah Univesity of Science and Technology, Feed the Future Innovation Lab for Applied Wheat Genomics through the U.S. Agency for International Development under the terms of Contract No AID-OAA-A-13-00051, and the Canadian Triticum Applied Genomics research project (CTAG2) funded by Genome Canada, Western Grain Research Foundation, Government of Saskatchewan, Saskatchewan Wheat Development Commission, Alberta Wheat Commission, Manitoba Crop Alliance, DuPont Pioneer, Viterra, and Secan. Kansas Wheat Commission; Canadian Triticum Applied Genomics research project (CTAG2); United States Agency for International Development, Grant/Award Number: AID-OAA-A-13-00051; King Abdullah University of Science and Technology, Grant/Award Number: BAS/1/1098-01-01.

ASJC Scopus subject areas

Genetics
Agronomy and Crop Science
Plant Science

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10.1002/tpg2.20309

https://repository.kaust.edu.sa/bitstream/10754/691444/1/The%20Plant%20Genome%20-%202023%20-%20Shrestha%20-%20Wheat%20doubled%20haploids%20have%20a%20marked%20prevalence%20of%20chromosomal%20aberrations.pdf

Cite this

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title = "Wheat doubled haploids have a marked prevalence of chromosomal aberrations",

abstract = "Double haploid (DH) population development is widely used in many crops, including wheat (Triticum aestivum L.), to rapidly produce fixed germplasm for breeding and genetic studies. The genome shock that takes place during DH induction could induce chromosomal aberrations that can impact genome integrity and subsequently plant fitness and agronomic performance. To evaluate the extent of chromosomal aberrations that exist as a result of the DH process, we studied two wheat DH populations: CDC Stanley×CDC Landmark and KS13H9×SYMonument. We utilized high-throughput skim sequencing to construct digital karyotypes of these populations to quantify deletions and aneuploidy with high resolution and accuracy, which was confirmed in selected plants by cytological analysis. The two populations studied showed high proportion of abnormal primary DH lines, 55 and 45%, respectively, based on at least one abnormality per progeny. The chromosomal abnormalities are genetically unstable and were observed segregating in the subsequent generations. These observations have important implications for the use of DH lines in genetics and breeding.",

author = "Sandesh Shrestha and Dal-Hoe Koo and Byron Evers and Shuangye Wu and Sean Walkowiak and Pierre Hucl and Curtis Pozniak and Fritz, {Allan K.} and Jesse Poland",

note = "KAUST Repository Item: Exported on 2023-05-05 Acknowledged KAUST grant number(s): BAS/1/1098-01-01 Acknowledgements: This project was completed with support from the Kansas Wheat Commission and the Kansas Wheat Alliance, King Abdullah Univesity of Science and Technology, Feed the Future Innovation Lab for Applied Wheat Genomics through the U.S. Agency for International Development under the terms of Contract No AID-OAA-A-13-00051, and the Canadian Triticum Applied Genomics research project (CTAG2) funded by Genome Canada, Western Grain Research Foundation, Government of Saskatchewan, Saskatchewan Wheat Development Commission, Alberta Wheat Commission, Manitoba Crop Alliance, DuPont Pioneer, Viterra, and Secan. Kansas Wheat Commission; Canadian Triticum Applied Genomics research project (CTAG2); United States Agency for International Development, Grant/Award Number: AID-OAA-A-13-00051; King Abdullah University of Science and Technology, Grant/Award Number: BAS/1/1098-01-01.",

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language = "English (US)",

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AU - Shrestha, Sandesh

AU - Koo, Dal-Hoe

AU - Evers, Byron

AU - Wu, Shuangye

AU - Walkowiak, Sean

AU - Hucl, Pierre

AU - Pozniak, Curtis

AU - Fritz, Allan K.

AU - Poland, Jesse

N1 - KAUST Repository Item: Exported on 2023-05-05 Acknowledged KAUST grant number(s): BAS/1/1098-01-01 Acknowledgements: This project was completed with support from the Kansas Wheat Commission and the Kansas Wheat Alliance, King Abdullah Univesity of Science and Technology, Feed the Future Innovation Lab for Applied Wheat Genomics through the U.S. Agency for International Development under the terms of Contract No AID-OAA-A-13-00051, and the Canadian Triticum Applied Genomics research project (CTAG2) funded by Genome Canada, Western Grain Research Foundation, Government of Saskatchewan, Saskatchewan Wheat Development Commission, Alberta Wheat Commission, Manitoba Crop Alliance, DuPont Pioneer, Viterra, and Secan. Kansas Wheat Commission; Canadian Triticum Applied Genomics research project (CTAG2); United States Agency for International Development, Grant/Award Number: AID-OAA-A-13-00051; King Abdullah University of Science and Technology, Grant/Award Number: BAS/1/1098-01-01.

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N2 - Double haploid (DH) population development is widely used in many crops, including wheat (Triticum aestivum L.), to rapidly produce fixed germplasm for breeding and genetic studies. The genome shock that takes place during DH induction could induce chromosomal aberrations that can impact genome integrity and subsequently plant fitness and agronomic performance. To evaluate the extent of chromosomal aberrations that exist as a result of the DH process, we studied two wheat DH populations: CDC Stanley×CDC Landmark and KS13H9×SYMonument. We utilized high-throughput skim sequencing to construct digital karyotypes of these populations to quantify deletions and aneuploidy with high resolution and accuracy, which was confirmed in selected plants by cytological analysis. The two populations studied showed high proportion of abnormal primary DH lines, 55 and 45%, respectively, based on at least one abnormality per progeny. The chromosomal abnormalities are genetically unstable and were observed segregating in the subsequent generations. These observations have important implications for the use of DH lines in genetics and breeding.

AB - Double haploid (DH) population development is widely used in many crops, including wheat (Triticum aestivum L.), to rapidly produce fixed germplasm for breeding and genetic studies. The genome shock that takes place during DH induction could induce chromosomal aberrations that can impact genome integrity and subsequently plant fitness and agronomic performance. To evaluate the extent of chromosomal aberrations that exist as a result of the DH process, we studied two wheat DH populations: CDC Stanley×CDC Landmark and KS13H9×SYMonument. We utilized high-throughput skim sequencing to construct digital karyotypes of these populations to quantify deletions and aneuploidy with high resolution and accuracy, which was confirmed in selected plants by cytological analysis. The two populations studied showed high proportion of abnormal primary DH lines, 55 and 45%, respectively, based on at least one abnormality per progeny. The chromosomal abnormalities are genetically unstable and were observed segregating in the subsequent generations. These observations have important implications for the use of DH lines in genetics and breeding.

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SN - 1940-3372

JO - The Plant Genome

JF - The Plant Genome

ER -

Wheat doubled haploids have a marked prevalence of chromosomal aberrations

Abstract

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