Wavefront engineering microscopy to study 3D mechanotransduction in living cells

Valentina Emiliani; Dan Cojoc; Enrico Ferrari; Valeria Garbin; Christiane Durieux; Enzo Di Fabrizio

doi:10.1117/12.662531

Wavefront engineering microscopy to study 3D mechanotransduction in living cells

Valentina Emiliani^*, Dan Cojoc, Enrico Ferrari, Valeria Garbin, Christiane Durieux, Enzo Di Fabrizio

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

We present an experimental system based on the use of a spatial light modulator which enables to perform simultaneously 3D optical manipulation and optical sectioning. This has been achieved by modifying the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. We demonstrated the capability of the system in two experimental schemes, in a first one we performed a 3D optical scanning of 6 trapped beads by displacing the beads through a fixed imaging plane. In a second one we scan the imaging plane and simultaneously compensate for the movement of the objective in order to keep the trapping plane at a fixed position.

Original language	English (US)
Title of host publication	Nanophotonics
DOIs	https://doi.org/10.1117/12.662531
State	Published - 2006
Externally published	Yes
Event	Nanophotonics - Strasbourg, France Duration: Apr 3 2006 → Apr 5 2006

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	6195
ISSN (Print)	0277-786X

Other

Other	Nanophotonics
Country/Territory	France
City	Strasbourg
Period	04/3/06 → 04/5/06

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.662531

Cite this

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title = "Wavefront engineering microscopy to study 3D mechanotransduction in living cells",

abstract = "We present an experimental system based on the use of a spatial light modulator which enables to perform simultaneously 3D optical manipulation and optical sectioning. This has been achieved by modifying the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. We demonstrated the capability of the system in two experimental schemes, in a first one we performed a 3D optical scanning of 6 trapped beads by displacing the beads through a fixed imaging plane. In a second one we scan the imaging plane and simultaneously compensate for the movement of the objective in order to keep the trapping plane at a fixed position.",

author = "Valentina Emiliani and Dan Cojoc and Enrico Ferrari and Valeria Garbin and Christiane Durieux and {Di Fabrizio}, Enzo",

year = "2006",

doi = "10.1117/12.662531",

language = "English (US)",

isbn = "0819462519",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

booktitle = "Nanophotonics",

note = "Nanophotonics ; Conference date: 03-04-2006 Through 05-04-2006",

}

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T1 - Wavefront engineering microscopy to study 3D mechanotransduction in living cells

AU - Emiliani, Valentina

AU - Cojoc, Dan

AU - Ferrari, Enrico

AU - Garbin, Valeria

AU - Durieux, Christiane

AU - Di Fabrizio, Enzo

PY - 2006

Y1 - 2006

N2 - We present an experimental system based on the use of a spatial light modulator which enables to perform simultaneously 3D optical manipulation and optical sectioning. This has been achieved by modifying the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. We demonstrated the capability of the system in two experimental schemes, in a first one we performed a 3D optical scanning of 6 trapped beads by displacing the beads through a fixed imaging plane. In a second one we scan the imaging plane and simultaneously compensate for the movement of the objective in order to keep the trapping plane at a fixed position.

AB - We present an experimental system based on the use of a spatial light modulator which enables to perform simultaneously 3D optical manipulation and optical sectioning. This has been achieved by modifying the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. We demonstrated the capability of the system in two experimental schemes, in a first one we performed a 3D optical scanning of 6 trapped beads by displacing the beads through a fixed imaging plane. In a second one we scan the imaging plane and simultaneously compensate for the movement of the objective in order to keep the trapping plane at a fixed position.

UR - http://www.scopus.com/inward/record.url?scp=33746687888&partnerID=8YFLogxK

U2 - 10.1117/12.662531

DO - 10.1117/12.662531

M3 - Conference contribution

AN - SCOPUS:33746687888

SN - 0819462519

SN - 9780819462510

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Nanophotonics

T2 - Nanophotonics

Y2 - 3 April 2006 through 5 April 2006

ER -

Wavefront engineering microscopy to study 3D mechanotransduction in living cells

Abstract

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