Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids

Jianing Mi; Juan C. Moreno; Yagiz Alagoz; Kit Xi Liew; Aparna Balakrishna; Xiongjie Zheng; Salim Al-Babili

doi:10.1016/bs.mie.2021.10.012

Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids

Jianing Mi, Juan C. Moreno, Yagiz Alagoz, Kit Xi Liew, Aparna Balakrishna, Xiongjie Zheng, Salim Al-Babili^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

5 Scopus citations

Abstract

Apocarotenoids (APOs) are a class of carotenoid oxidation products with high structural and functional diversity. Apart from serving as precursors of phytohormones, fungal pheromones and vitamin A, several APOs act as signaling molecules involved in stress response and growth as regulators in plants. To comprehensively profile plant APOs, we established an improved ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical platform. The improved APO analytical platform consists of an optimized sequential APO sample preparation and multiple UHPLC-MS detection methods and was successfully used to identify and quantify multiple subclasses of APOs from tomato fruits. By integrating ultrasound-assisted extraction, solid phase extraction, and chemical derivatization, the improved sequential APOs sample preparation facilitates the simultaneous preparation of different subclasses of APOs from plant materials. In addition, multiple UHPLC-MS detection methods enables high throughput analysis of APOs. Application of this analytical strategy can make important contributions to understanding the function of these compounds and significantly facilitate the elucidation of plant APO metabolism.

Original language	English (US)
Title of host publication	Carotenoids
Subtitle of host publication	Carotenoid and Apocarotenoid Analysis
Editors	Eleanore T. Wurtzel
Publisher	Academic Press Inc.
Pages	285-309
Number of pages	25
ISBN (Print)	9780323999755
DOIs	https://doi.org/10.1016/bs.mie.2021.10.012
State	Published - Jan 2022

Publication series

Name	Methods in Enzymology
Volume	670
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

Bibliographical note

Funding Information:
This work was supported by baseline funding and a Competitive Research Grant (CRG4 and CRG5) given to Salim Al-Babili from the King Abdullah University of Science and Technology (KAUST).

Publisher Copyright:
© 2022 Elsevier Inc.

Keywords

Apocarotenoids
Diapocarotenals
Glycosylated apocarotenoids
Tomato
Ultra-high performance liquid chromatography-mass spectrometry

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.1016/bs.mie.2021.10.012

Cite this

Mi, J., Moreno, J. C., Alagoz, Y., Liew, K. X., Balakrishna, A., Zheng, X., & Al-Babili, S. (2022). Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids. In E. T. Wurtzel (Ed.), Carotenoids: Carotenoid and Apocarotenoid Analysis (pp. 285-309). (Methods in Enzymology; Vol. 670). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2021.10.012

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abstract = "Apocarotenoids (APOs) are a class of carotenoid oxidation products with high structural and functional diversity. Apart from serving as precursors of phytohormones, fungal pheromones and vitamin A, several APOs act as signaling molecules involved in stress response and growth as regulators in plants. To comprehensively profile plant APOs, we established an improved ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical platform. The improved APO analytical platform consists of an optimized sequential APO sample preparation and multiple UHPLC-MS detection methods and was successfully used to identify and quantify multiple subclasses of APOs from tomato fruits. By integrating ultrasound-assisted extraction, solid phase extraction, and chemical derivatization, the improved sequential APOs sample preparation facilitates the simultaneous preparation of different subclasses of APOs from plant materials. In addition, multiple UHPLC-MS detection methods enables high throughput analysis of APOs. Application of this analytical strategy can make important contributions to understanding the function of these compounds and significantly facilitate the elucidation of plant APO metabolism.",

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Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids. / Mi, Jianing; Moreno, Juan C.; Alagoz, Yagiz et al.
Carotenoids: Carotenoid and Apocarotenoid Analysis. ed. / Eleanore T. Wurtzel. Academic Press Inc., 2022. p. 285-309 (Methods in Enzymology; Vol. 670).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

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