Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites

Paolo Galli; Giovanni Strona; Anna Maria Villa; Francesca Benzoni; Fabrizio Stefani; Silvia Maria Doglia; Delane C. Kritsky

doi:10.1016/j.ijpara.2006.11.017

Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites

Paolo Galli, Giovanni Strona, Anna Maria Villa, Francesca Benzoni, Fabrizio Stefani, Silvia Maria Doglia, Delane C. Kritsky

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A new method of three-dimensional (3-D) analysis of sclerotised structures of monogenoids was performed by processing z-series images using 3D-Doctor. Z-series were obtained from Gomori's trichrome-stained specimens of marine and freshwater monogenoids under laser scanning confocal fluorescence microscopy. Measurements obtained from 3-D images were then compared with those from 2-D images taken from both flattened and unflattened specimens. Data comparison demonstrated that 3-D morphometry allowed avoidance of over-estimation due to deformation and the reduction of errors associated with different spatial orientations. Moreover, study of 3-D images permitted observation of morphological details that are not detectable in 2-D representations. © 2006 Australian Society for Parasitology Inc.

Original language	English (US)
Pages (from-to)	449-456
Number of pages	8
Journal	International Journal for Parasitology
Volume	37
Issue number	3-4
DOIs	https://doi.org/10.1016/j.ijpara.2006.11.017
State	Published - Mar 1 2007
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-09-25

ASJC Scopus subject areas

Infectious Diseases
Parasitology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijpara.2006.11.017

Cite this

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title = "Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites",

abstract = "A new method of three-dimensional (3-D) analysis of sclerotised structures of monogenoids was performed by processing z-series images using 3D-Doctor. Z-series were obtained from Gomori's trichrome-stained specimens of marine and freshwater monogenoids under laser scanning confocal fluorescence microscopy. Measurements obtained from 3-D images were then compared with those from 2-D images taken from both flattened and unflattened specimens. Data comparison demonstrated that 3-D morphometry allowed avoidance of over-estimation due to deformation and the reduction of errors associated with different spatial orientations. Moreover, study of 3-D images permitted observation of morphological details that are not detectable in 2-D representations. {\textcopyright} 2006 Australian Society for Parasitology Inc.",

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AU - Galli, Paolo

AU - Strona, Giovanni

AU - Villa, Anna Maria

AU - Benzoni, Francesca

AU - Stefani, Fabrizio

AU - Doglia, Silvia Maria

AU - Kritsky, Delane C.

N1 - Generated from Scopus record by KAUST IRTS on 2023-09-25

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ER -

Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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