Tuber borchii mycelial protoplasts isolation, characterization and functional delivery of liposome content, a new step towards truffles biotechnology

Anna Poma; Sabrina Colafarina; Tania Limongi; Giovanni Pacioni

doi:10.1016/j.femsle.2005.10.003

Tuber borchii mycelial protoplasts isolation, characterization and functional delivery of liposome content, a new step towards truffles biotechnology

Anna Poma^*, Sabrina Colafarina, Tania Limongi, Giovanni Pacioni

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The filametous ascomycete Tuber borchii is a plant-symbiotic ectomycorrhizal microrganism with an high value due to the production of hypogeous fruitbodies (truffles). The present work was undertaken to develop a procedure for the release of T. borchii viable protoplasts from Tuber mycelium, isolate ATTC 96540; several factors which affect the isolation, morphology and viability were examined and developed in order to improve applications of T. borchii protoplasts in morphological, biochemical and genetic investigations (protoplast fusion or transformation). Functional delivery of liposome content into T. borchii protoplasts has also been examined with a cytotoxic ribosome inactivator as saporin. T. borchii protoplasts incubation/fusion with saporin containing liposomes were made to demonstrate the absence of cell wall of 16 days cultured protoplasts.

Original language	English (US)
Pages (from-to)	331-337
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	253
Issue number	2
DOIs	https://doi.org/10.1016/j.femsle.2005.10.003
State	Published - Dec 15 2005
Externally published	Yes

Bibliographical note

Funding Information:
We are grateful to Dr. S. Arcioni and his team (CNR Perugia) for his help in initial phases of this work and to Dr. A. Tagliola for her technical assistance. This work was supported by a special grant from CNR (Consiglio Nazionale delle Ricerche, Italy) and Regional Administrations for “Biotecnologia dei funghi eduli ectomicorrizici: dalle applicazioni agro-forestali a quelle agro-alimentari”.

Keywords

Liposomes
Mycorrhizal fungi
Protoplasts
Tuber borchii

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/j.femsle.2005.10.003

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title = "Tuber borchii mycelial protoplasts isolation, characterization and functional delivery of liposome content, a new step towards truffles biotechnology",

abstract = "The filametous ascomycete Tuber borchii is a plant-symbiotic ectomycorrhizal microrganism with an high value due to the production of hypogeous fruitbodies (truffles). The present work was undertaken to develop a procedure for the release of T. borchii viable protoplasts from Tuber mycelium, isolate ATTC 96540; several factors which affect the isolation, morphology and viability were examined and developed in order to improve applications of T. borchii protoplasts in morphological, biochemical and genetic investigations (protoplast fusion or transformation). Functional delivery of liposome content into T. borchii protoplasts has also been examined with a cytotoxic ribosome inactivator as saporin. T. borchii protoplasts incubation/fusion with saporin containing liposomes were made to demonstrate the absence of cell wall of 16 days cultured protoplasts.",

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AU - Pacioni, Giovanni

N1 - Funding Information: We are grateful to Dr. S. Arcioni and his team (CNR Perugia) for his help in initial phases of this work and to Dr. A. Tagliola for her technical assistance. This work was supported by a special grant from CNR (Consiglio Nazionale delle Ricerche, Italy) and Regional Administrations for “Biotecnologia dei funghi eduli ectomicorrizici: dalle applicazioni agro-forestali a quelle agro-alimentari”.

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Tuber borchii mycelial protoplasts isolation, characterization and functional delivery of liposome content, a new step towards truffles biotechnology

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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