Abstract
The filametous ascomycete Tuber borchii is a plant-symbiotic ectomycorrhizal microrganism with an high value due to the production of hypogeous fruitbodies (truffles). The present work was undertaken to develop a procedure for the release of T. borchii viable protoplasts from Tuber mycelium, isolate ATTC 96540; several factors which affect the isolation, morphology and viability were examined and developed in order to improve applications of T. borchii protoplasts in morphological, biochemical and genetic investigations (protoplast fusion or transformation). Functional delivery of liposome content into T. borchii protoplasts has also been examined with a cytotoxic ribosome inactivator as saporin. T. borchii protoplasts incubation/fusion with saporin containing liposomes were made to demonstrate the absence of cell wall of 16 days cultured protoplasts.
Original language | English (US) |
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Pages (from-to) | 331-337 |
Number of pages | 7 |
Journal | FEMS Microbiology Letters |
Volume | 253 |
Issue number | 2 |
DOIs | |
State | Published - Dec 15 2005 |
Externally published | Yes |
Bibliographical note
Funding Information:We are grateful to Dr. S. Arcioni and his team (CNR Perugia) for his help in initial phases of this work and to Dr. A. Tagliola for her technical assistance. This work was supported by a special grant from CNR (Consiglio Nazionale delle Ricerche, Italy) and Regional Administrations for “Biotecnologia dei funghi eduli ectomicorrizici: dalle applicazioni agro-forestali a quelle agro-alimentari”.
Keywords
- Liposomes
- Mycorrhizal fungi
- Protoplasts
- Tuber borchii
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics