Transposon-assisted genetic engineering with mos1- mediated single-copy insertion (MosSCI)

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

15 Scopus citations


Transgenesis in model organisms is necessary to determine the function, expression, and subcellular localization of gene products. In Caenorhabditis elegans, injected DNA can be propagated as multicopy extrachromosomal arrays but transgenes in arrays are mosaic, over-expressed in some tissues and silenced in the germline. Here, a method to insert a transgene into a specific genomic location called Mos1- mediated single-copy insertion (MosSCI) is described. Single-copy insertion allows transgene expression at levels that approximate endogenous gene expression as well as expression in the germline.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2015
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.


  • Endogenous levels of gene expression
  • Germline expression
  • Mos1 single-copy insertion (MosSCI)
  • Mos1 transposon
  • Transgenesis
  • Universal insertion sites

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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