Transposon-assisted genetic engineering with mos1- mediated single-copy insertion (MosSCI)

Christian Frøkjær-Jensen

doi:10.1007/978-1-4939-2842-2_5

Transposon-assisted genetic engineering with mos1- mediated single-copy insertion (MosSCI)

Christian Frøkjær-Jensen

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

16 Scopus citations

Abstract

Transgenesis in model organisms is necessary to determine the function, expression, and subcellular localization of gene products. In Caenorhabditis elegans, injected DNA can be propagated as multicopy extrachromosomal arrays but transgenes in arrays are mosaic, over-expressed in some tissues and silenced in the germline. Here, a method to insert a transgene into a specific genomic location called Mos1- mediated single-copy insertion (MosSCI) is described. Single-copy insertion allows transgene expression at levels that approximate endogenous gene expression as well as expression in the germline.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	49-58
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-2842-2_5
State	Published - 2015
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1327
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

Endogenous levels of gene expression
Germline expression
Mos1 single-copy insertion (MosSCI)
Mos1 transposon
Transgenesis
Universal insertion sites

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-2842-2_5

Cite this

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abstract = "Transgenesis in model organisms is necessary to determine the function, expression, and subcellular localization of gene products. In Caenorhabditis elegans, injected DNA can be propagated as multicopy extrachromosomal arrays but transgenes in arrays are mosaic, over-expressed in some tissues and silenced in the germline. Here, a method to insert a transgene into a specific genomic location called Mos1- mediated single-copy insertion (MosSCI) is described. Single-copy insertion allows transgene expression at levels that approximate endogenous gene expression as well as expression in the germline.",

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AB - Transgenesis in model organisms is necessary to determine the function, expression, and subcellular localization of gene products. In Caenorhabditis elegans, injected DNA can be propagated as multicopy extrachromosomal arrays but transgenes in arrays are mosaic, over-expressed in some tissues and silenced in the germline. Here, a method to insert a transgene into a specific genomic location called Mos1- mediated single-copy insertion (MosSCI) is described. Single-copy insertion allows transgene expression at levels that approximate endogenous gene expression as well as expression in the germline.

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KW - Germline expression

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KW - Universal insertion sites

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Transposon-assisted genetic engineering with mos1- mediated single-copy insertion (MosSCI)

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

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