Towards the development of a supercritical carbon dioxide spray process to coat solid protein particles

Miao Yu; Ahmad S. Sediq; Shiduo Zhang; M. Reza Nejadnik; Hayley A. Every; Wim Jiskoot; Geert Jan Witkamp

doi:10.1016/j.supflu.2017.12.014

Towards the development of a supercritical carbon dioxide spray process to coat solid protein particles

Miao Yu^*, Ahmad S. Sediq, Shiduo Zhang, M. Reza Nejadnik, Hayley A. Every, Wim Jiskoot, Geert Jan Witkamp

^*Corresponding author for this work

Environmental Science and Engineering

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The aim of this study was to develop a supercritical carbon dioxide (scCO₂) spray process to coat solid protein particles with a hydrophilic polymer. The final purpose is to manufacture drug particles exhibiting controlled release behaviour in patients. Lysozyme microparticles (about 20 μm) were suspended in a vessel into which a dextran sulphate (DS) solution was dispersed by scCO₂ via a nozzle. Upon interaction with the droplets, DS was deposited onto or mixed with suspended lysozyme particles. Particles of about 100 μm were obtained. The zeta-potential analysis and elemental analysis indicated that the top layer of the particles consisted of both lysozyme and DS. Some of the produced particulate materials showed retarded lysozyme release when exposed to water or phosphate buffered saline, holding promise for future production of controlled drug delivery systems for therapeutic proteins.

Original language	English (US)
Pages (from-to)	49-59
Number of pages	11
Journal	Journal of Supercritical Fluids
Volume	141
DOIs	https://doi.org/10.1016/j.supflu.2017.12.014
State	Published - Nov 2018

Bibliographical note

Publisher Copyright:
© 2017 Elsevier B.V.

Keywords

Dextran sulphate
Drug delivery systems
Lysozyme
Proteins
Supercritical carbon dioxide

ASJC Scopus subject areas

General Chemical Engineering
Condensed Matter Physics
Physical and Theoretical Chemistry

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10.1016/j.supflu.2017.12.014

Cite this

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title = "Towards the development of a supercritical carbon dioxide spray process to coat solid protein particles",

abstract = "The aim of this study was to develop a supercritical carbon dioxide (scCO2) spray process to coat solid protein particles with a hydrophilic polymer. The final purpose is to manufacture drug particles exhibiting controlled release behaviour in patients. Lysozyme microparticles (about 20 μm) were suspended in a vessel into which a dextran sulphate (DS) solution was dispersed by scCO2 via a nozzle. Upon interaction with the droplets, DS was deposited onto or mixed with suspended lysozyme particles. Particles of about 100 μm were obtained. The zeta-potential analysis and elemental analysis indicated that the top layer of the particles consisted of both lysozyme and DS. Some of the produced particulate materials showed retarded lysozyme release when exposed to water or phosphate buffered saline, holding promise for future production of controlled drug delivery systems for therapeutic proteins.",

keywords = "Dextran sulphate, Drug delivery systems, Lysozyme, Proteins, Supercritical carbon dioxide",

author = "Miao Yu and Sediq, {Ahmad S.} and Shiduo Zhang and Nejadnik, {M. Reza} and Every, {Hayley A.} and Wim Jiskoot and Witkamp, {Geert Jan}",

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year = "2018",

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doi = "10.1016/j.supflu.2017.12.014",

language = "English (US)",

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AU - Yu, Miao

AU - Sediq, Ahmad S.

AU - Zhang, Shiduo

AU - Nejadnik, M. Reza

AU - Every, Hayley A.

AU - Jiskoot, Wim

AU - Witkamp, Geert Jan

PY - 2018/11

Y1 - 2018/11

N2 - The aim of this study was to develop a supercritical carbon dioxide (scCO2) spray process to coat solid protein particles with a hydrophilic polymer. The final purpose is to manufacture drug particles exhibiting controlled release behaviour in patients. Lysozyme microparticles (about 20 μm) were suspended in a vessel into which a dextran sulphate (DS) solution was dispersed by scCO2 via a nozzle. Upon interaction with the droplets, DS was deposited onto or mixed with suspended lysozyme particles. Particles of about 100 μm were obtained. The zeta-potential analysis and elemental analysis indicated that the top layer of the particles consisted of both lysozyme and DS. Some of the produced particulate materials showed retarded lysozyme release when exposed to water or phosphate buffered saline, holding promise for future production of controlled drug delivery systems for therapeutic proteins.

AB - The aim of this study was to develop a supercritical carbon dioxide (scCO2) spray process to coat solid protein particles with a hydrophilic polymer. The final purpose is to manufacture drug particles exhibiting controlled release behaviour in patients. Lysozyme microparticles (about 20 μm) were suspended in a vessel into which a dextran sulphate (DS) solution was dispersed by scCO2 via a nozzle. Upon interaction with the droplets, DS was deposited onto or mixed with suspended lysozyme particles. Particles of about 100 μm were obtained. The zeta-potential analysis and elemental analysis indicated that the top layer of the particles consisted of both lysozyme and DS. Some of the produced particulate materials showed retarded lysozyme release when exposed to water or phosphate buffered saline, holding promise for future production of controlled drug delivery systems for therapeutic proteins.

KW - Dextran sulphate

KW - Drug delivery systems

KW - Lysozyme

KW - Proteins

KW - Supercritical carbon dioxide

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U2 - 10.1016/j.supflu.2017.12.014

DO - 10.1016/j.supflu.2017.12.014

M3 - Article

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SN - 0896-8446

VL - 141

SP - 49

EP - 59

JO - Journal of Supercritical Fluids

JF - Journal of Supercritical Fluids

ER -

Towards the development of a supercritical carbon dioxide spray process to coat solid protein particles

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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