Tomography using multiple wavelengths in digital holography: Method, simulations and experiments

Frédéric Montfort; Florian Charrière; Tristan Colomb; Jonas Kuehn; Etienne Cuche; Christian Depeursinge

doi:10.1117/12.663253

Tomography using multiple wavelengths in digital holography: Method, simulations and experiments

Frédéric Montfort, Florian Charrière, Tristan Colomb, Jonas Kuehn, Etienne Cuche, Christian Depeursinge^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

In this paper we present a method for tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording at different wavelengths equally separated in the k-domain, in off-axis geometry, of the interference between a reference wave and an object wave reflected by a microscopic sample and magnified by a microscope objective. A couple charged device (CCD) camera records consecutively the resulting holograms, which are then numerically reconstructed to obtain their resulting wavefront. Those wavefronts are then summed. The result of this operation is a constructive addition of complex waves in the selected plane and destructive addition in the others. Varying the plane of interest enables the scan the object in depth. For the presented simulations and experiments, twenty wavelengths are used in the 480-700 nm range. An object consisting of irregularly stairs with heights of 375, 525, 975, 1200 and 1275 nm is reconstructed. Its lateral dimensions are 250 × 250 microns. The results show clearly a 3D imaging technique with axial resolution under the micron.

Original language	English (US)
Title of host publication	Optical Micro- and Nanometrology in Microsystems Technology
Volume	6188
DOIs	https://doi.org/10.1117/12.663253
State	Published - 2006
Externally published	Yes
Event	Optical Micro- and Nanometrology in Microsystems Technology - Strasbourg, France Duration: Apr 5 2006 → Apr 7 2006

Other

Other	Optical Micro- and Nanometrology in Microsystems Technology
Country/Territory	France
City	Strasbourg
Period	04/5/06 → 04/7/06

Keywords

Digital holography
Image reconstruction technique
Microscopy
Three-dimensional image acquisition
Tomography

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.663253

Cite this

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title = "Tomography using multiple wavelengths in digital holography: Method, simulations and experiments",

abstract = "In this paper we present a method for tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording at different wavelengths equally separated in the k-domain, in off-axis geometry, of the interference between a reference wave and an object wave reflected by a microscopic sample and magnified by a microscope objective. A couple charged device (CCD) camera records consecutively the resulting holograms, which are then numerically reconstructed to obtain their resulting wavefront. Those wavefronts are then summed. The result of this operation is a constructive addition of complex waves in the selected plane and destructive addition in the others. Varying the plane of interest enables the scan the object in depth. For the presented simulations and experiments, twenty wavelengths are used in the 480-700 nm range. An object consisting of irregularly stairs with heights of 375, 525, 975, 1200 and 1275 nm is reconstructed. Its lateral dimensions are 250 × 250 microns. The results show clearly a 3D imaging technique with axial resolution under the micron.",

keywords = "Digital holography, Image reconstruction technique, Microscopy, Three-dimensional image acquisition, Tomography",

author = "Fr{\'e}d{\'e}ric Montfort and Florian Charri{\`e}re and Tristan Colomb and Jonas Kuehn and Etienne Cuche and Christian Depeursinge",

year = "2006",

doi = "10.1117/12.663253",

language = "English (US)",

isbn = "0819462446",

volume = "6188",

booktitle = "Optical Micro- and Nanometrology in Microsystems Technology",

note = "Optical Micro- and Nanometrology in Microsystems Technology ; Conference date: 05-04-2006 Through 07-04-2006",

}

Montfort, F, Charrière, F, Colomb, T, Kuehn, J, Cuche, E & Depeursinge, C 2006, Tomography using multiple wavelengths in digital holography: Method, simulations and experiments. in Optical Micro- and Nanometrology in Microsystems Technology. vol. 6188, 618802, Optical Micro- and Nanometrology in Microsystems Technology, Strasbourg, France, 04/5/06. https://doi.org/10.1117/12.663253

TY - GEN

T1 - Tomography using multiple wavelengths in digital holography

T2 - Optical Micro- and Nanometrology in Microsystems Technology

AU - Montfort, Frédéric

AU - Charrière, Florian

AU - Colomb, Tristan

AU - Kuehn, Jonas

AU - Cuche, Etienne

AU - Depeursinge, Christian

PY - 2006

Y1 - 2006

N2 - In this paper we present a method for tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording at different wavelengths equally separated in the k-domain, in off-axis geometry, of the interference between a reference wave and an object wave reflected by a microscopic sample and magnified by a microscope objective. A couple charged device (CCD) camera records consecutively the resulting holograms, which are then numerically reconstructed to obtain their resulting wavefront. Those wavefronts are then summed. The result of this operation is a constructive addition of complex waves in the selected plane and destructive addition in the others. Varying the plane of interest enables the scan the object in depth. For the presented simulations and experiments, twenty wavelengths are used in the 480-700 nm range. An object consisting of irregularly stairs with heights of 375, 525, 975, 1200 and 1275 nm is reconstructed. Its lateral dimensions are 250 × 250 microns. The results show clearly a 3D imaging technique with axial resolution under the micron.

AB - In this paper we present a method for tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording at different wavelengths equally separated in the k-domain, in off-axis geometry, of the interference between a reference wave and an object wave reflected by a microscopic sample and magnified by a microscope objective. A couple charged device (CCD) camera records consecutively the resulting holograms, which are then numerically reconstructed to obtain their resulting wavefront. Those wavefronts are then summed. The result of this operation is a constructive addition of complex waves in the selected plane and destructive addition in the others. Varying the plane of interest enables the scan the object in depth. For the presented simulations and experiments, twenty wavelengths are used in the 480-700 nm range. An object consisting of irregularly stairs with heights of 375, 525, 975, 1200 and 1275 nm is reconstructed. Its lateral dimensions are 250 × 250 microns. The results show clearly a 3D imaging technique with axial resolution under the micron.

KW - Digital holography

KW - Image reconstruction technique

KW - Microscopy

KW - Three-dimensional image acquisition

KW - Tomography

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U2 - 10.1117/12.663253

DO - 10.1117/12.663253

M3 - Conference contribution

AN - SCOPUS:33746718788

SN - 0819462446

SN - 9780819462442

VL - 6188

BT - Optical Micro- and Nanometrology in Microsystems Technology

Y2 - 5 April 2006 through 7 April 2006

ER -

Tomography using multiple wavelengths in digital holography: Method, simulations and experiments

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Keywords

ASJC Scopus subject areas

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