Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape

Kholod Alamoudi, Patricia Martins, Jonas G. Croissant, Sachin Patil, Haneen Omar, Niveen M. Khashab

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Improving the delivery of siRNA into cancer cells via bubble liposomes. Designing a thermoresponsive pegylated liposome through the introduction of ammonium bicarbonate salt into liposomes so as to control their endosomal escape for gene therapy.A sub-200 nm nanovector was fully characterized and examined for cellular uptake, cytotoxicity, endosomal escape and gene silencing.The siRNA-liposomes were internalized into cancer cells within 5 min and then released siRNAs in the cytosol prior to lysosomal degradation upon external temperature elevation. This was confirmed by confocal bioimaging and gene silencing reaching up to 90% and further demonstrated by the protein inhibition of both target genes.The thermoresponsiveness of ammonium bicarbonate containing liposomes enabled the rapid endosomal escape of the particles and resulted in an efficient gene silencing.
Original languageEnglish (US)
Pages (from-to)1421-1433
Number of pages13
Issue number12
StatePublished - May 19 2017

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We gratefully acknowledge support from King Abdullah University of Science and Technology (KAUST). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.


Dive into the research topics of 'Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape'. Together they form a unique fingerprint.

Cite this