The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

Pierre Jacob; Gwilherm Brisou; Marion Dalmais; Johanne Thévenin; Froukje van der Wal; David Latrasse; Ravi Suresh Devani; Moussa Benhamed; Bertrand Dubreucq; Adnane Boualem; Loic Lepiniec; Richard G. H. Immink; Heribert Hirt; Abdelhafid Bendahmane

doi:10.3390/genes12050746

The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

Pierre Jacob, Gwilherm Brisou, Marion Dalmais, Johanne Thévenin, Froukje van der Wal, David Latrasse, Ravi Suresh Devani, Moussa Benhamed, Bertrand Dubreucq, Adnane Boualem, Loic Lepiniec, Richard G. H. Immink, Heribert Hirt, Abdelhafid Bendahmane

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.

Original language	English (US)
Pages (from-to)	746
Journal	Genes
Volume	12
Issue number	5
DOIs	https://doi.org/10.3390/genes12050746
State	Published - May 15 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-05-25
Acknowledgements: We thank P. Audigier for plant handling, and are grateful for the cell biology imaging platform and research facilities provided by the Institute of Plant-Science Paris-Saclay (IPS2).

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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https://repository.kaust.edu.sa/bitstream/10754/669225/1/genes-12-00746.pdf

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@article{2011fe6b7ba3423d8adfa0ad60bcaf12,

title = "The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis",

abstract = "HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.",

author = "Pierre Jacob and Gwilherm Brisou and Marion Dalmais and Johanne Th{\'e}venin and {van der Wal}, Froukje and David Latrasse and {Suresh Devani}, Ravi and Moussa Benhamed and Bertrand Dubreucq and Adnane Boualem and Loic Lepiniec and Immink, {Richard G. H.} and Heribert Hirt and Abdelhafid Bendahmane",

note = "KAUST Repository Item: Exported on 2021-05-25 Acknowledgements: We thank P. Audigier for plant handling, and are grateful for the cell biology imaging platform and research facilities provided by the Institute of Plant-Science Paris-Saclay (IPS2).",

year = "2021",

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doi = "10.3390/genes12050746",

language = "English (US)",

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journal = "Genes",

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T1 - The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

AU - Jacob, Pierre

AU - Brisou, Gwilherm

AU - Dalmais, Marion

AU - Thévenin, Johanne

AU - van der Wal, Froukje

AU - Latrasse, David

AU - Suresh Devani, Ravi

AU - Benhamed, Moussa

AU - Dubreucq, Bertrand

AU - Boualem, Adnane

AU - Lepiniec, Loic

AU - Immink, Richard G. H.

AU - Hirt, Heribert

AU - Bendahmane, Abdelhafid

N1 - KAUST Repository Item: Exported on 2021-05-25 Acknowledgements: We thank P. Audigier for plant handling, and are grateful for the cell biology imaging platform and research facilities provided by the Institute of Plant-Science Paris-Saclay (IPS2).

PY - 2021/5/15

Y1 - 2021/5/15

N2 - HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.

AB - HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.

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DO - 10.3390/genes12050746

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SN - 2073-4425

VL - 12

SP - 746

JO - Genes

JF - Genes

IS - 5

ER -

The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

Abstract

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