Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymatic activity of recombinant CD45, LAR, PTP1B phosphatases and cellular CD45 in Jurkat cells. The obtained results show that ferrous iron (II) is potent inhibitor of CD45, LAR and PTP1B, but the inhibitory effect is concentration dependent. We found that the higher concentrations of ferrous iron (II) increase the inactivation of CD45, LAR and PTP1B phosphatase caused by hydrogen peroxide, but the addition of the physiological concentration (500 nM) of ferrous iron (II) has even a slightly preventive effect on the phosphatase activity against hydrogen peroxide.
|Original language||English (US)|
|Number of pages||12|
|State||Published - Dec 1 2015|
Bibliographical noteFunding Information:
This work was supported by MN Grant No. 01-0172/08/259 from Medical University of Gdansk and Ministry of Science and Higher Education. MG gratefully acknowledges to Polish National Science Center Grant No. 2012/07/N/NZ1/00012. MW acknowledges ST46 from Medical University of Gdansk. JAT acknowledges support from NSERC (Canada) and the Allard Foundation.
© 2015 The Author(s).
- Ferrous iron
- Hydrogen peroxide
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
- Metals and Alloys