The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases

Alicja Kuban-Jankowska; Magdalena Gorska; Lukasz Jaremko; Mariusz Jaremko; Jack A. Tuszynski; Michal Wozniak

doi:10.1007/s10534-015-9882-4

The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases

Alicja Kuban-Jankowska^*, Magdalena Gorska, Lukasz Jaremko, Mariusz Jaremko, Jack A. Tuszynski, Michal Wozniak

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymatic activity of recombinant CD45, LAR, PTP1B phosphatases and cellular CD45 in Jurkat cells. The obtained results show that ferrous iron (II) is potent inhibitor of CD45, LAR and PTP1B, but the inhibitory effect is concentration dependent. We found that the higher concentrations of ferrous iron (II) increase the inactivation of CD45, LAR and PTP1B phosphatase caused by hydrogen peroxide, but the addition of the physiological concentration (500 nM) of ferrous iron (II) has even a slightly preventive effect on the phosphatase activity against hydrogen peroxide.

Original language	English (US)
Pages (from-to)	975-986
Number of pages	12
Journal	BioMetals
Volume	28
Issue number	6
DOIs	https://doi.org/10.1007/s10534-015-9882-4
State	Published - Dec 1 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 The Author(s).

Keywords

CD45
Ferrous iron
Hydrogen peroxide
LAR
PTP1B

ASJC Scopus subject areas

Biomaterials
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
Metals and Alloys

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10.1007/s10534-015-9882-4

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title = "The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases",

abstract = "Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymatic activity of recombinant CD45, LAR, PTP1B phosphatases and cellular CD45 in Jurkat cells. The obtained results show that ferrous iron (II) is potent inhibitor of CD45, LAR and PTP1B, but the inhibitory effect is concentration dependent. We found that the higher concentrations of ferrous iron (II) increase the inactivation of CD45, LAR and PTP1B phosphatase caused by hydrogen peroxide, but the addition of the physiological concentration (500 nM) of ferrous iron (II) has even a slightly preventive effect on the phosphatase activity against hydrogen peroxide.",

keywords = "CD45, Ferrous iron, Hydrogen peroxide, LAR, PTP1B",

author = "Alicja Kuban-Jankowska and Magdalena Gorska and Lukasz Jaremko and Mariusz Jaremko and Tuszynski, {Jack A.} and Michal Wozniak",

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year = "2015",

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doi = "10.1007/s10534-015-9882-4",

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T1 - The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases

AU - Kuban-Jankowska, Alicja

AU - Gorska, Magdalena

AU - Jaremko, Lukasz

AU - Jaremko, Mariusz

AU - Tuszynski, Jack A.

AU - Wozniak, Michal

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymatic activity of recombinant CD45, LAR, PTP1B phosphatases and cellular CD45 in Jurkat cells. The obtained results show that ferrous iron (II) is potent inhibitor of CD45, LAR and PTP1B, but the inhibitory effect is concentration dependent. We found that the higher concentrations of ferrous iron (II) increase the inactivation of CD45, LAR and PTP1B phosphatase caused by hydrogen peroxide, but the addition of the physiological concentration (500 nM) of ferrous iron (II) has even a slightly preventive effect on the phosphatase activity against hydrogen peroxide.

AB - Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymatic activity of recombinant CD45, LAR, PTP1B phosphatases and cellular CD45 in Jurkat cells. The obtained results show that ferrous iron (II) is potent inhibitor of CD45, LAR and PTP1B, but the inhibitory effect is concentration dependent. We found that the higher concentrations of ferrous iron (II) increase the inactivation of CD45, LAR and PTP1B phosphatase caused by hydrogen peroxide, but the addition of the physiological concentration (500 nM) of ferrous iron (II) has even a slightly preventive effect on the phosphatase activity against hydrogen peroxide.

KW - CD45

KW - Ferrous iron

KW - Hydrogen peroxide

KW - LAR

KW - PTP1B

UR - http://www.scopus.com/inward/record.url?scp=84946564434&partnerID=8YFLogxK

U2 - 10.1007/s10534-015-9882-4

DO - 10.1007/s10534-015-9882-4

M3 - Article

C2 - 26407665

AN - SCOPUS:84946564434

SN - 0966-0844

VL - 28

SP - 975

EP - 986

JO - BioMetals

JF - BioMetals

IS - 6

ER -

The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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