The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis

Markus Teige; Elisabeth Scheikl; Thomas Eulgem; Róbert Dóczi; Kazuya Ichimura; Kazuo Shinozaki; Jeffery L. Dangl; Heribert Hirt

doi:10.1016/j.molcel.2004.06.023

The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis

Markus Teige^*, Elisabeth Scheikl, Thomas Eulgem, Róbert Dóczi, Kazuya Ichimura, Kazuo Shinozaki, Jeffery L. Dangl, Heribert Hirt

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

817 Scopus citations

Abstract

The Arabidopsis mitogen-activated protein kinase (MAPK) kinase 2 (MKK2) and the downstream MAPKs MPK4 and MPK6 were isolated by functional complementation of osmosensitive yeast mutants. In Arabidopsis protoplasts, MKK2 was specifically activated by cold and salt stress and by the stress-induced MAPK kinase kinase MEKK1. Yeast two-hybrid, in vitro, and in vivo protein kinase assays revealed that MKK2 directly targets MPK4 and MPK6. Accordingly, plants overexpressing MKK2 exhibited constitutive MPK4 and MPK6 activity, constitutively upregulated expression of stress-induced marker genes, and increased freezing and salt tolerance. In contrast, mkk2 null plants were impaired in MPK4 and MPK6 activation and were hypersensitive to salt and cold stress. Full genome transcriptome analysis of MKK2-overexpressing plants demonstrated altered expression of 152 genes involved in transcriptional regulation, signal transduction, cellular defense, and stress metabolism. These data identify a MAP kinase signaling cascade mediating cold and salt stress tolerance in plants.

Original language	English (US)
Pages (from-to)	141-152
Number of pages	12
Journal	Molecular cell
Volume	15
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2004.06.023
State	Published - Jul 2 2004
Externally published	Yes

Bibliographical note

Funding Information:
We thank G. Ammerer and all members of the Hirt laboratory for helpful suggestions and critical reading of the manuscript, A. Auer and A. Belokurow for technical assistance, and F. Lacroute for providing an Arabidopsis cDNA library for yeast complementation. This work was supported by grants from the Austrian Science Foundation to H.H. and a Training and Mobility for Researchers (TMR) fellowship (to M.T.) from the European Community. T.E. was supported by Deutsche Forschungsgemeinschaft (DFG; EU 51/1) and Max-Planck-Society-Otto Hahn Medallion postdoctoral fellowships. Work in the J.L.D. lab was supported by USDA-NRI CSREES 2002-35301-12059.

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Access to Document

10.1016/j.molcel.2004.06.023

Cite this

@article{c48667471b1249e39a4bac360e236b0b,

title = "The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis",

abstract = "The Arabidopsis mitogen-activated protein kinase (MAPK) kinase 2 (MKK2) and the downstream MAPKs MPK4 and MPK6 were isolated by functional complementation of osmosensitive yeast mutants. In Arabidopsis protoplasts, MKK2 was specifically activated by cold and salt stress and by the stress-induced MAPK kinase kinase MEKK1. Yeast two-hybrid, in vitro, and in vivo protein kinase assays revealed that MKK2 directly targets MPK4 and MPK6. Accordingly, plants overexpressing MKK2 exhibited constitutive MPK4 and MPK6 activity, constitutively upregulated expression of stress-induced marker genes, and increased freezing and salt tolerance. In contrast, mkk2 null plants were impaired in MPK4 and MPK6 activation and were hypersensitive to salt and cold stress. Full genome transcriptome analysis of MKK2-overexpressing plants demonstrated altered expression of 152 genes involved in transcriptional regulation, signal transduction, cellular defense, and stress metabolism. These data identify a MAP kinase signaling cascade mediating cold and salt stress tolerance in plants.",

author = "Markus Teige and Elisabeth Scheikl and Thomas Eulgem and R{\'o}bert D{\'o}czi and Kazuya Ichimura and Kazuo Shinozaki and Dangl, {Jeffery L.} and Heribert Hirt",

note = "Funding Information: We thank G. Ammerer and all members of the Hirt laboratory for helpful suggestions and critical reading of the manuscript, A. Auer and A. Belokurow for technical assistance, and F. Lacroute for providing an Arabidopsis cDNA library for yeast complementation. This work was supported by grants from the Austrian Science Foundation to H.H. and a Training and Mobility for Researchers (TMR) fellowship (to M.T.) from the European Community. T.E. was supported by Deutsche Forschungsgemeinschaft (DFG; EU 51/1) and Max-Planck-Society-Otto Hahn Medallion postdoctoral fellowships. Work in the J.L.D. lab was supported by USDA-NRI CSREES 2002-35301-12059. ",

year = "2004",

month = jul,

day = "2",

doi = "10.1016/j.molcel.2004.06.023",

language = "English (US)",

volume = "15",

pages = "141--152",

journal = "Molecular cell",

issn = "1097-2765",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis

AU - Teige, Markus

AU - Scheikl, Elisabeth

AU - Eulgem, Thomas

AU - Dóczi, Róbert

AU - Ichimura, Kazuya

AU - Shinozaki, Kazuo

AU - Dangl, Jeffery L.

AU - Hirt, Heribert

N1 - Funding Information: We thank G. Ammerer and all members of the Hirt laboratory for helpful suggestions and critical reading of the manuscript, A. Auer and A. Belokurow for technical assistance, and F. Lacroute for providing an Arabidopsis cDNA library for yeast complementation. This work was supported by grants from the Austrian Science Foundation to H.H. and a Training and Mobility for Researchers (TMR) fellowship (to M.T.) from the European Community. T.E. was supported by Deutsche Forschungsgemeinschaft (DFG; EU 51/1) and Max-Planck-Society-Otto Hahn Medallion postdoctoral fellowships. Work in the J.L.D. lab was supported by USDA-NRI CSREES 2002-35301-12059.

PY - 2004/7/2

Y1 - 2004/7/2

N2 - The Arabidopsis mitogen-activated protein kinase (MAPK) kinase 2 (MKK2) and the downstream MAPKs MPK4 and MPK6 were isolated by functional complementation of osmosensitive yeast mutants. In Arabidopsis protoplasts, MKK2 was specifically activated by cold and salt stress and by the stress-induced MAPK kinase kinase MEKK1. Yeast two-hybrid, in vitro, and in vivo protein kinase assays revealed that MKK2 directly targets MPK4 and MPK6. Accordingly, plants overexpressing MKK2 exhibited constitutive MPK4 and MPK6 activity, constitutively upregulated expression of stress-induced marker genes, and increased freezing and salt tolerance. In contrast, mkk2 null plants were impaired in MPK4 and MPK6 activation and were hypersensitive to salt and cold stress. Full genome transcriptome analysis of MKK2-overexpressing plants demonstrated altered expression of 152 genes involved in transcriptional regulation, signal transduction, cellular defense, and stress metabolism. These data identify a MAP kinase signaling cascade mediating cold and salt stress tolerance in plants.

AB - The Arabidopsis mitogen-activated protein kinase (MAPK) kinase 2 (MKK2) and the downstream MAPKs MPK4 and MPK6 were isolated by functional complementation of osmosensitive yeast mutants. In Arabidopsis protoplasts, MKK2 was specifically activated by cold and salt stress and by the stress-induced MAPK kinase kinase MEKK1. Yeast two-hybrid, in vitro, and in vivo protein kinase assays revealed that MKK2 directly targets MPK4 and MPK6. Accordingly, plants overexpressing MKK2 exhibited constitutive MPK4 and MPK6 activity, constitutively upregulated expression of stress-induced marker genes, and increased freezing and salt tolerance. In contrast, mkk2 null plants were impaired in MPK4 and MPK6 activation and were hypersensitive to salt and cold stress. Full genome transcriptome analysis of MKK2-overexpressing plants demonstrated altered expression of 152 genes involved in transcriptional regulation, signal transduction, cellular defense, and stress metabolism. These data identify a MAP kinase signaling cascade mediating cold and salt stress tolerance in plants.

UR - http://www.scopus.com/inward/record.url?scp=3042801230&partnerID=8YFLogxK

U2 - 10.1016/j.molcel.2004.06.023

DO - 10.1016/j.molcel.2004.06.023

M3 - Article

C2 - 15225555

AN - SCOPUS:3042801230

SN - 1097-2765

VL - 15

SP - 141

EP - 152

JO - Molecular cell

JF - Molecular cell

IS - 1

ER -

The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this