The glycolytic shift in fumarate-hydratase-deficient kidney cancer lowers AMPK levels, increases anabolic propensities and lowers cellular iron levels

Winghang Tong, Carole Sourbier, Gennadiy Kovtunovych, Suhyoung Jeong, Manish A. Vira, Manik Chandra Ghosh, Vladimir Valera Romero, Rachid Sougrat, Sophie Vaulont, Benoît Viollet, Yeongsang Kim, Sunmin Lee, Jane B. Trepel, Ramaprasad Srinivasan, Gennady Bratslavsky, Youfeng Yang, William Marston Linehan, Tracey A. Rouault

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152 Scopus citations


Inactivation of the TCA cycle enzyme, fumarate hydratase (FH), drives a metabolic shift to aerobic glycolysis in FH-deficient kidney tumors and cell lines from patients with hereditary leiomyomatosis renal cell cancer (HLRCC), resulting in decreased levels of AMP-activated kinase (AMPK) and p53 tumor suppressor, and activation of the anabolic factors, acetyl-CoA carboxylase and ribosomal protein S6. Reduced AMPK levels lead to diminished expression of the DMT1 iron transporter, and the resulting cytosolic iron deficiency activates the iron regulatory proteins, IRP1 and IRP2, and increases expression of the hypoxia inducible factor HIF-1α, but not HIF-2α. Silencing of HIF-1α or activation of AMPK diminishes invasive activities, indicating that alterations of HIF-1α and AMPK contribute to the oncogenic growth of FH-deficient cells. © 2011 Elsevier Inc.
Original languageEnglish (US)
Pages (from-to)315-327
Number of pages13
JournalCancer Cell
Issue number3
StatePublished - Sep 11 2011

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research
  • Oncology

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