The genome sequence of the wild tomato solanum pimpinellifolium provides insights into salinity tolerance

Rozaimi Razali, Salim Bougouffa, Mitchell J.L. Morton, Damien J. Lightfoot, Intikhab Alam, Magbubah Essack, Stefan T. Arold, Allan A. Kamau, Sandra M. Schmöckel, Yveline Pailles, Mohammed Shahid, Craig T. Michell, Salim Al-Babili, Yung Shwen Ho, Mark Tester, Vladimir B. Bajic*, Sónia Negrão

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Solanum pimpinellifolium, a wild relative of cultivated tomato, offers a wealth of breeding potential for desirable traits such as tolerance to abiotic and biotic stresses. Here, we report the genome assembly and annotation of S. pimpinellifolium ‘LA0480.’ Moreover, we present phenotypic data from one field experiment that demonstrate a greater salinity tolerance for fruit- and yield-related traits in S. pimpinellifolium compared with cultivated tomato. The ‘LA0480’ genome assembly size (811 Mb) and the number of annotated genes (25,970) are within the range observed for other sequenced tomato species. We developed and utilized the Dragon Eukaryotic Analyses Platform (DEAP) to functionally annotate the ‘LA0480’ protein-coding genes. Additionally, we used DEAP to compare protein function between S. pimpinellifolium and cultivated tomato. Our data suggest enrichment in genes involved in biotic and abiotic stress responses. To understand the genomic basis for these differences in S. pimpinellifolium and S. lycopersicum, we analyzed 15 genes that have previously been shown to mediate salinity tolerance in plants. We show that S. pimpinellifolium has a higher copy number of the inositol-3-phosphate synthase and phosphatase genes, which are both key enzymes in the production of inositol and its derivatives. Moreover, our analysis indicates that changes occurring in the inositol phosphate pathway may contribute to the observed higher salinity tolerance in ‘LA0480.’ Altogether, our work provides essential resources to understand and unlock the genetic and breeding potential of S. pimpinellifolium, and to discover the genomic basis underlying its environmental robustness.

Original languageEnglish (US)
Article number1402
JournalFrontiers in Plant Science
Volume9
DOIs
StatePublished - Oct 4 2018

Bibliographical note

Funding Information:
This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. 2302-01-01 and KAUST Base Research Funds to VB Grant No. BAS/1/1606-01-01 and to MT Grant No. BAS/1/1038-01-01.

Publisher Copyright:
© 2018 Razali, Bougouffa, Morton, Lightfoot, Alam, Essack, Arold, Kamau, Schmöckel, Pailles, Shahid, Michell, Al-Babili, Ho, Tester, Bajic and Negrão.

Keywords

  • Genome analysis
  • Inositol 3-phosphate synthase
  • Salinity tolerance
  • Solanum pimpinellifolium
  • Wild tomato

ASJC Scopus subject areas

  • Plant Science

Fingerprint

Dive into the research topics of 'The genome sequence of the wild tomato solanum pimpinellifolium provides insights into salinity tolerance'. Together they form a unique fingerprint.

Cite this