The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells

Yun Xia, Ignacio Sancho-Martinez, Emmanuel Nivet, Concepcion Rodriguez Esteban, Josep Maria Campistol, Juan Carlos Izpisua Belmonte*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

This protocol presents recently developed methodologies for the differentiation of human pluripotent stem cells (hPSCs) into ureteric bud (UB) progenitor-like cells. Differentiation of human PSCs to UB progenitor-like cells allows for the generation of chimeric kidney cultures in which the human cells can self-assemble into chimeric 3D structures in combination with embryonic mouse kidney cells over a period of 18 d. UB progenitor-like cells are generated by a two-step process that combines in vitro commitment of human PSCs, whether embryonic stem cells (ESCs) or induced PSCs (iPSCs), under chemically defined culture conditions, with ex vivo cultures for the induction of 3D organogenesis. The models described here provide new opportunities for investigating human kidney development, modeling disease, evaluating regenerative medicine strategies, as well as for toxicology studies.

Original languageEnglish (US)
Pages (from-to)2693-2704
Number of pages12
JournalNature Protocols
Volume9
Issue number11
DOIs
StatePublished - Nov 27 2014
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014 Nature America, Inc. All rights reserved.

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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