The function of the hypusine-containing proteins of yeast and other eukaryotes is well conserved

Viktor Magdolen, Hannelore Klier, Thorsten Wöhl, Friedrich Klink, Heribert Hirt, Joachim Hauber, Friedrich Lottspeich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


The hypusine-containing protein (Hypp) is highly conserved in evolution, from man to archaebacteria, but is not found in eubacteria. Hypp is essential for the viability for yeast cells, where two forms are encoded by the genes HYP1 and HYP2. The hypusine-containing protein Hyp2p, encoded by the HYP2 gene in yeast, is present under both aerobic and anaerobic conditions, whereas Hyp1p synthesis is restricted to anaerobiosis. hyp1 disruption mutants grown under anaerobic conditions reveal no detectable alteration in phenotype relative to wild-type strains. We demonstrate that either Hyp1p or Hyp2p alone is sufficient for normal growth under both metabolic conditions. Moreover, Hypp from various eukaryotic species (slime mold, alfalfa and man) carries the lysine to hypusine modification when expressed in yeast and can substitute functionally for Hyp2p in strains disrupted for HYP2, indicating a highly conserved function of this protein. In contrast, the archaebacterial Hypp expressed in yeast is neither modified by hypusine, nor does it allow growth of cells deficient for yeast Hypp.

Original languageEnglish (US)
Pages (from-to)646-652
Number of pages7
JournalMgg Molecular & General Genetics
Issue number6
StatePublished - Nov 1994
Externally publishedYes


  • Anaerobiosis
  • Eukaryotic translation initiation factor eIF-5A
  • Saccharomyces cerevisiae Complementation analysis

ASJC Scopus subject areas

  • Genetics


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