The expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells

Jian Li; Brigitte Happ; Christian Schetter; Cornelia Oellig; Charlotte Hauser; Kazumichi Kuroda; Dagmar Knebel-Mörsdorf; Hans Dieter Klenk; Walter Doerfler

doi:10.1016/0378-1135(90)90137-K

The expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells

Jian Li^*, Brigitte Happ, Christian Schetter, Cornelia Oellig, Charlotte Hauser, Kazumichi Kuroda, Dagmar Knebel-Mörsdorf, Hans Dieter Klenk, Walter Doerfler

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

This report presents a synopsis of recently published work in our laboratory on the molecular biology of the insect baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV). The following studies have been summarized. 1. (1) On the mode of transcription of the AcNPV genome in insect cells. 2. (2) Translation of proteins encoded in the 8.12 to 85.0 map unit segment of AcNPV. 3. (3) Inserts of insect cell DNA in the AcNPV genome. 4. (4) Expression of influenza (fowl plague) virus haemagglutinin in Spodoptera frugiperda insect cells, and successful immunization of chickens. 5. (5) Synthesis of the influenza virus haemagglutinin in insect larvae by recombinant AcNPV. This insect virus system will continue to serve as a model for research on the molecular biology of insects. Moreover, the baculovirus system has been recognized as a very efficient and safe eukaryotic expression vector.

Original language	English (US)
Pages (from-to)	73-78
Number of pages	6
Journal	Veterinary Microbiology
Volume	23
Issue number	1-4
DOIs	https://doi.org/10.1016/0378-1135(90)90137-K
State	Published - Jun 1990
Externally published	Yes

ASJC Scopus subject areas

Microbiology
General Veterinary

Access to Document

10.1016/0378-1135(90)90137-K

Cite this

@article{f983a46500344ac5b3ba8c2cd001be3c,

title = "The expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells",

abstract = "This report presents a synopsis of recently published work in our laboratory on the molecular biology of the insect baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV). The following studies have been summarized. 1. (1) On the mode of transcription of the AcNPV genome in insect cells. 2. (2) Translation of proteins encoded in the 8.12 to 85.0 map unit segment of AcNPV. 3. (3) Inserts of insect cell DNA in the AcNPV genome. 4. (4) Expression of influenza (fowl plague) virus haemagglutinin in Spodoptera frugiperda insect cells, and successful immunization of chickens. 5. (5) Synthesis of the influenza virus haemagglutinin in insect larvae by recombinant AcNPV. This insect virus system will continue to serve as a model for research on the molecular biology of insects. Moreover, the baculovirus system has been recognized as a very efficient and safe eukaryotic expression vector.",

author = "Jian Li and Brigitte Happ and Christian Schetter and Cornelia Oellig and Charlotte Hauser and Kazumichi Kuroda and Dagmar Knebel-M{\"o}rsdorf and Klenk, {Hans Dieter} and Walter Doerfler",

year = "1990",

month = jun,

doi = "10.1016/0378-1135(90)90137-K",

language = "English (US)",

volume = "23",

pages = "73--78",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier",

number = "1-4",

}

TY - JOUR

T1 - The expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells

AU - Li, Jian

AU - Happ, Brigitte

AU - Schetter, Christian

AU - Oellig, Cornelia

AU - Hauser, Charlotte

AU - Kuroda, Kazumichi

AU - Knebel-Mörsdorf, Dagmar

AU - Klenk, Hans Dieter

AU - Doerfler, Walter

PY - 1990/6

Y1 - 1990/6

N2 - This report presents a synopsis of recently published work in our laboratory on the molecular biology of the insect baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV). The following studies have been summarized. 1. (1) On the mode of transcription of the AcNPV genome in insect cells. 2. (2) Translation of proteins encoded in the 8.12 to 85.0 map unit segment of AcNPV. 3. (3) Inserts of insect cell DNA in the AcNPV genome. 4. (4) Expression of influenza (fowl plague) virus haemagglutinin in Spodoptera frugiperda insect cells, and successful immunization of chickens. 5. (5) Synthesis of the influenza virus haemagglutinin in insect larvae by recombinant AcNPV. This insect virus system will continue to serve as a model for research on the molecular biology of insects. Moreover, the baculovirus system has been recognized as a very efficient and safe eukaryotic expression vector.

AB - This report presents a synopsis of recently published work in our laboratory on the molecular biology of the insect baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV). The following studies have been summarized. 1. (1) On the mode of transcription of the AcNPV genome in insect cells. 2. (2) Translation of proteins encoded in the 8.12 to 85.0 map unit segment of AcNPV. 3. (3) Inserts of insect cell DNA in the AcNPV genome. 4. (4) Expression of influenza (fowl plague) virus haemagglutinin in Spodoptera frugiperda insect cells, and successful immunization of chickens. 5. (5) Synthesis of the influenza virus haemagglutinin in insect larvae by recombinant AcNPV. This insect virus system will continue to serve as a model for research on the molecular biology of insects. Moreover, the baculovirus system has been recognized as a very efficient and safe eukaryotic expression vector.

UR - http://www.scopus.com/inward/record.url?scp=0025120537&partnerID=8YFLogxK

U2 - 10.1016/0378-1135(90)90137-K

DO - 10.1016/0378-1135(90)90137-K

M3 - Article

C2 - 2205973

AN - SCOPUS:0025120537

SN - 0378-1135

VL - 23

SP - 73

EP - 78

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1-4

ER -

The expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this