Understanding and controlling cell proliferation on biomaterial surfaces is critical for scaffold/artificial-niche design in tissue engineering. The mechanism by which underlying integrin ligates with functionalized biomaterials to induce cell proliferation is still not completely understood. In this study, poly-l-lactide (PL) scaffold surfaces were functionalized using layers of cerium oxide nanoparticles (CNPs), which have recently attracted attention for use in therapeutic application due to their catalytic ability of Ce4+ and Ce3+ sites. To isolate the influence of Ce valance states of CNPs on cell proliferation, human mesenchymal stem cells (hMSCs) and osteoblast-like cells (MG63) were cultured on the PL/CNP surfaces with dominant Ce4+ and Ce3+ regions. Despite cell type (hMSCs and MG63 cells), different surface features of Ce4+ and Ce3+ regions clearly promoted and inhibited cell spreading, migration and adhesion behavior, resulting in rapid and slow cell proliferation, respectively. Cell proliferation results of various modified CNPs with different surface charge and hydrophobicity/hydrophilicity, indicate that Ce valence states closely correlated with the specific cell morphologies and cell-material interactions that trigger cell proliferation. This finding suggests that the cell-material interactions, which influence cell proliferation, may be controlled by introduction of metal elements with different valence states onto the biomaterial surface. © 2014 Elsevier Ltd.
|Original language||English (US)|
|Number of pages||13|
|State||Published - May 2014|
Bibliographical noteKAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported, in part, by the World Premier International (WPI) Research Center Initiative Program. Technical support by the Bio-Organic Materials Facility in the Nanotechnology Innovation Station, NIMS, Japan is acknowledged. The authors thank Ms. H. Morita and Dr. X. LI for technical support and suggestions about biological experiments, Ms. S. Kajiwara for technical support of confocal microscope observations (Nanotechnology Innovation Station, NIMS, Japan). We would like to thank Prof. T. Aoyagi (International Center for Materials Nanoarchitectonics, NIMS, Japan) and Dr. L Ghibelli (University of Rome Tor Vergata) for invaluable discussions.
ASJC Scopus subject areas
- Mechanics of Materials
- Ceramics and Composites