The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis

Hyungsae Kim, Sungjin Kim, Nazia Abbasi, Ray Anthony Bressan, Daejin Yun, Sangdong Yoo, SukYun Kwon, Sangbong Choi

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67 Scopus citations


Dof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxialabaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5′-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxialabaxial polarity. © 2010 Blackwell Publishing Ltd.
Original languageEnglish (US)
Pages (from-to)524-535
Number of pages12
JournalThe Plant Journal
Issue number3
StatePublished - Oct 5 2010

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We thank Gyung-Tae Kim for the rev-6 seeds and Yul-Ho Kim for the SEM pictures. S.-B. Choi and S.-Y. Kwon were supported by Bio-Green21 (grant 20080401034045 from the Rural Development Administration), and S.-B. Choi (CG2151) and S.-Y. Kwon (CG2120-1) were supported by the Crop Functional Genomics Center funded by the Ministry of Education, Science and Technology, Korea. S.-D. Yoo is supported by NRF (2010-0016898). R.A. Bressan and D.-J. Yun are supported by the WCU program (grant R32-10148).

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics


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