TY - JOUR
T1 - The chromosome-level genome of double-petal phenotype jasmine (Jasminum sambac Aiton) provides insights into the biosynthesis of floral scent
AU - Qi, Xiangyu
AU - Wang, Huadi
AU - Liu, Shuyun
AU - Chen, Shuangshuang
AU - Feng, Jing
AU - Chen, Huijie
AU - Qin, Ziyi
AU - Chen, Quanming
AU - Blilou, Ikram
AU - Deng, Yanming
N1 - KAUST Repository Item: Exported on 2023-05-10
Acknowledgements: This work was financially supported by the National Natural Science Foundation of China (Grant No. 31772338), and the Basic Scientific Research Business Special Project of Jiangsu Academy of Agricultural Sciences (Grant No. 0090756100ZX). We thank the staffs of the Central Laboratory at Jiangsu Academy of Agricultural Sciences for their help in HS-SPME and GC–MS analysis.
PY - 2023/3/16
Y1 - 2023/3/16
N2 - Jasmine (Jasminum sambac Aiton) is a well-known cultivated plant species for its fragrant flowers used in the perfume industry and cosmetics. However, the genetic basis of its floral scent is largely unknown. In this study, using PacBio, Illumina, 10× Genomics and high-throughput chromosome conformation capture (Hi-C) sequencing technologies, a high-quality chromosome-level reference genome for J. sambac was obtained, exploiting a double-petal phenotype cultivar ‘Shuangbanmoli’ (JSSB). The results showed that the final assembled genome of JSSB is 580.33 Mb in size (contig N50 = 1.05 Mb; scaffold N50 = 45.07 Mb) with a total of 39 618 predicted protein-coding genes. Our analyses revealed that the JSSB genome has undergone an ancient whole-genome duplication (WGD) event at 91.68 million years ago (Mya). It was estimated that J. sambac diverged from the lineage leading to Olea europaea and Osmanthus fragrans about 28.8 Mya. On the basis of a combination of genomic, transcriptomic and metabolomic analyses, a range of floral scent volatiles and genes were identified involved in the benzenoid/phenylpropanoid and terpenoid biosynthesis pathways. The results provide new insights into the molecular mechanism of its fragrance biosynthesis in jasmine.
AB - Jasmine (Jasminum sambac Aiton) is a well-known cultivated plant species for its fragrant flowers used in the perfume industry and cosmetics. However, the genetic basis of its floral scent is largely unknown. In this study, using PacBio, Illumina, 10× Genomics and high-throughput chromosome conformation capture (Hi-C) sequencing technologies, a high-quality chromosome-level reference genome for J. sambac was obtained, exploiting a double-petal phenotype cultivar ‘Shuangbanmoli’ (JSSB). The results showed that the final assembled genome of JSSB is 580.33 Mb in size (contig N50 = 1.05 Mb; scaffold N50 = 45.07 Mb) with a total of 39 618 predicted protein-coding genes. Our analyses revealed that the JSSB genome has undergone an ancient whole-genome duplication (WGD) event at 91.68 million years ago (Mya). It was estimated that J. sambac diverged from the lineage leading to Olea europaea and Osmanthus fragrans about 28.8 Mya. On the basis of a combination of genomic, transcriptomic and metabolomic analyses, a range of floral scent volatiles and genes were identified involved in the benzenoid/phenylpropanoid and terpenoid biosynthesis pathways. The results provide new insights into the molecular mechanism of its fragrance biosynthesis in jasmine.
UR - http://hdl.handle.net/10754/691598
UR - https://linkinghub.elsevier.com/retrieve/pii/S2468014123000456
UR - http://www.scopus.com/inward/record.url?scp=85151913258&partnerID=8YFLogxK
U2 - 10.1016/j.hpj.2023.03.006
DO - 10.1016/j.hpj.2023.03.006
M3 - Article
SN - 2095-9885
JO - Horticultural Plant Journal
JF - Horticultural Plant Journal
ER -