TY - JOUR
T1 - The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output
AU - Stijf-Bultsma, Yvette
AU - Sommer, Lilly
AU - Tauber, Maria
AU - Baalbaki, Mai
AU - Giardoglou, Panagiota
AU - Jones, David R.
AU - Gelato, Kathy A.
AU - vanPelt, Jason
AU - Shah, Zahid
AU - Rahnamoun, Homa
AU - Toma, Clara
AU - Anderson, Karen E.
AU - Hawkins, Philip
AU - Lauberth, Shannon M.
AU - Haramis, Anna Pavlina G.
AU - Hart, Daniel
AU - Fischle, Wolfgang
AU - Divecha, Nullin
N1 - Publisher Copyright:
© 2015 The Authors.
PY - 2015/5/7
Y1 - 2015/5/7
N2 - Phosphoinositides (PI) are important signaling molecules in the nucleus that influence gene expression. However, if and how nuclear PI directly affects the transcriptional machinery is not known. We report that the lipid kinase PIP4K2B regulates nuclear PI5P and the expression of myogenic genes during myoblast differentiation. A targeted screen for PI interactors identified the PHD finger of TAF3, a TATA box binding protein-associated factor with important roles in transcription regulation, pluripotency, and differentiation. We show that the PI interaction site is distinct from the known H3K4me3 binding region of TAF3 and that PI binding modulates association of TAF3 with H3K4me3 invitro and with chromatin invivo. Analysis of TAF3 mutants indicates that TAF3 transduces PIP4K2B-mediated alterations in PI into changes in specific gene transcription. Our study reveals TAF3 as a direct target of nuclear PI and further illustrates the importance of basal transcription components as signal transducers.
AB - Phosphoinositides (PI) are important signaling molecules in the nucleus that influence gene expression. However, if and how nuclear PI directly affects the transcriptional machinery is not known. We report that the lipid kinase PIP4K2B regulates nuclear PI5P and the expression of myogenic genes during myoblast differentiation. A targeted screen for PI interactors identified the PHD finger of TAF3, a TATA box binding protein-associated factor with important roles in transcription regulation, pluripotency, and differentiation. We show that the PI interaction site is distinct from the known H3K4me3 binding region of TAF3 and that PI binding modulates association of TAF3 with H3K4me3 invitro and with chromatin invivo. Analysis of TAF3 mutants indicates that TAF3 transduces PIP4K2B-mediated alterations in PI into changes in specific gene transcription. Our study reveals TAF3 as a direct target of nuclear PI and further illustrates the importance of basal transcription components as signal transducers.
UR - http://www.scopus.com/inward/record.url?scp=84928925874&partnerID=8YFLogxK
U2 - 10.1016/j.molcel.2015.03.009
DO - 10.1016/j.molcel.2015.03.009
M3 - Article
C2 - 25866244
AN - SCOPUS:84928925874
SN - 1097-2765
VL - 58
SP - 453
EP - 467
JO - Molecular cell
JF - Molecular cell
IS - 3
ER -