The Arabidopsis SWI/SNF protein BAF60 mediates seedling growth control by modulating DNA accessibility

Teddy Jégu; Alaguraj Veluchamy; Juan Sebastian Ramirez Prado; Charley Rizzi-Paillet; Magalie Perez; Anaïs Lhomme; David Latrasse; Emeline Coleno; Serge Vicaire; Stéphanie Legras; Bernard Jost; Martin Rougée; Fredy Barneche; Catherine Bergounioux; Martin Crespi; Magdy M. Mahfouz; Heribert Hirt; Cécile Raynaud; Moussa Benhamed

doi:10.1186/s13059-017-1246-7

The Arabidopsis SWI/SNF protein BAF60 mediates seedling growth control by modulating DNA accessibility

Teddy Jégu, Alaguraj Veluchamy, Juan Sebastian Ramirez Prado, Charley Rizzi-Paillet, Magalie Perez, Anaïs Lhomme, David Latrasse, Emeline Coleno, Serge Vicaire, Stéphanie Legras, Bernard Jost, Martin Rougée, Fredy Barneche, Catherine Bergounioux, Martin Crespi, Magdy M. Mahfouz, Heribert Hirt, Cécile Raynaud, Moussa Benhamed

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53 Scopus citations

Abstract

Plant adaptive responses to changing environments involve complex molecular interplays between intrinsic and external signals. Whilst much is known on the signaling components mediating diurnal, light, and temperature controls on plant development, their influence on chromatin-based transcriptional controls remains poorly explored.In this study we show that a SWI/SNF chromatin remodeler subunit, BAF60, represses seedling growth by modulating DNA accessibility of hypocotyl cell size regulatory genes. BAF60 binds nucleosome-free regions of multiple G box-containing genes, opposing in cis the promoting effect of the photomorphogenic and thermomorphogenic regulator Phytochrome Interacting Factor 4 (PIF4) on hypocotyl elongation. Furthermore, BAF60 expression level is regulated in response to light and daily rhythms.These results unveil a short path between a chromatin remodeler and a signaling component to fine-tune plant morphogenesis in response to environmental conditions.

Original language	English (US)
Journal	Genome biology
Volume	18
Issue number	1
DOIs	https://doi.org/10.1186/s13059-017-1246-7
State	Published - Jun 15 2017

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was funded by King Abdullah University of Science and Technology (KAUST) and Université Paris Sud. The IPS2 benefits from the support of the LabExSaclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS).

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https://repository.kaust.edu.sa/bitstream/10754/625066/1/art3A10.11862Fs13059-017-1246-7.pdf

Cite this

Jégu, T., Veluchamy, A., Ramirez Prado, J. S., Rizzi-Paillet, C., Perez, M., Lhomme, A., Latrasse, D., Coleno, E., Vicaire, S., Legras, S., Jost, B., Rougée, M., Barneche, F., Bergounioux, C., Crespi, M., Mahfouz, M. M., Hirt, H., Raynaud, C., & Benhamed, M. (2017). The Arabidopsis SWI/SNF protein BAF60 mediates seedling growth control by modulating DNA accessibility. Genome biology, 18(1). https://doi.org/10.1186/s13059-017-1246-7

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title = "The Arabidopsis SWI/SNF protein BAF60 mediates seedling growth control by modulating DNA accessibility",

abstract = "Plant adaptive responses to changing environments involve complex molecular interplays between intrinsic and external signals. Whilst much is known on the signaling components mediating diurnal, light, and temperature controls on plant development, their influence on chromatin-based transcriptional controls remains poorly explored.In this study we show that a SWI/SNF chromatin remodeler subunit, BAF60, represses seedling growth by modulating DNA accessibility of hypocotyl cell size regulatory genes. BAF60 binds nucleosome-free regions of multiple G box-containing genes, opposing in cis the promoting effect of the photomorphogenic and thermomorphogenic regulator Phytochrome Interacting Factor 4 (PIF4) on hypocotyl elongation. Furthermore, BAF60 expression level is regulated in response to light and daily rhythms.These results unveil a short path between a chromatin remodeler and a signaling component to fine-tune plant morphogenesis in response to environmental conditions.",

author = "Teddy J{\'e}gu and Alaguraj Veluchamy and {Ramirez Prado}, {Juan Sebastian} and Charley Rizzi-Paillet and Magalie Perez and Ana{\"i}s Lhomme and David Latrasse and Emeline Coleno and Serge Vicaire and St{\'e}phanie Legras and Bernard Jost and Martin Roug{\'e}e and Fredy Barneche and Catherine Bergounioux and Martin Crespi and Mahfouz, {Magdy M.} and Heribert Hirt and C{\'e}cile Raynaud and Moussa Benhamed",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was funded by King Abdullah University of Science and Technology (KAUST) and Universit{\'e} Paris Sud. The IPS2 benefits from the support of the LabExSaclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS).",

year = "2017",

month = jun,

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doi = "10.1186/s13059-017-1246-7",

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Jégu, T, Veluchamy, A, Ramirez Prado, JS, Rizzi-Paillet, C, Perez, M, Lhomme, A, Latrasse, D, Coleno, E, Vicaire, S, Legras, S, Jost, B, Rougée, M, Barneche, F, Bergounioux, C, Crespi, M, Mahfouz, MM , Hirt, H, Raynaud, C & Benhamed, M 2017, 'The Arabidopsis SWI/SNF protein BAF60 mediates seedling growth control by modulating DNA accessibility', Genome biology, vol. 18, no. 1. https://doi.org/10.1186/s13059-017-1246-7

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T1 - The Arabidopsis SWI/SNF protein BAF60 mediates seedling growth control by modulating DNA accessibility

AU - Jégu, Teddy

AU - Veluchamy, Alaguraj

AU - Ramirez Prado, Juan Sebastian

AU - Rizzi-Paillet, Charley

AU - Perez, Magalie

AU - Lhomme, Anaïs

AU - Latrasse, David

AU - Coleno, Emeline

AU - Vicaire, Serge

AU - Legras, Stéphanie

AU - Jost, Bernard

AU - Rougée, Martin

AU - Barneche, Fredy

AU - Bergounioux, Catherine

AU - Crespi, Martin

AU - Mahfouz, Magdy M.

AU - Hirt, Heribert

AU - Raynaud, Cécile

AU - Benhamed, Moussa

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was funded by King Abdullah University of Science and Technology (KAUST) and Université Paris Sud. The IPS2 benefits from the support of the LabExSaclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS).

PY - 2017/6/15

Y1 - 2017/6/15

N2 - Plant adaptive responses to changing environments involve complex molecular interplays between intrinsic and external signals. Whilst much is known on the signaling components mediating diurnal, light, and temperature controls on plant development, their influence on chromatin-based transcriptional controls remains poorly explored.In this study we show that a SWI/SNF chromatin remodeler subunit, BAF60, represses seedling growth by modulating DNA accessibility of hypocotyl cell size regulatory genes. BAF60 binds nucleosome-free regions of multiple G box-containing genes, opposing in cis the promoting effect of the photomorphogenic and thermomorphogenic regulator Phytochrome Interacting Factor 4 (PIF4) on hypocotyl elongation. Furthermore, BAF60 expression level is regulated in response to light and daily rhythms.These results unveil a short path between a chromatin remodeler and a signaling component to fine-tune plant morphogenesis in response to environmental conditions.

AB - Plant adaptive responses to changing environments involve complex molecular interplays between intrinsic and external signals. Whilst much is known on the signaling components mediating diurnal, light, and temperature controls on plant development, their influence on chromatin-based transcriptional controls remains poorly explored.In this study we show that a SWI/SNF chromatin remodeler subunit, BAF60, represses seedling growth by modulating DNA accessibility of hypocotyl cell size regulatory genes. BAF60 binds nucleosome-free regions of multiple G box-containing genes, opposing in cis the promoting effect of the photomorphogenic and thermomorphogenic regulator Phytochrome Interacting Factor 4 (PIF4) on hypocotyl elongation. Furthermore, BAF60 expression level is regulated in response to light and daily rhythms.These results unveil a short path between a chromatin remodeler and a signaling component to fine-tune plant morphogenesis in response to environmental conditions.

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UR - https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1246-7

UR - http://www.scopus.com/inward/record.url?scp=85021873247&partnerID=8YFLogxK

U2 - 10.1186/s13059-017-1246-7

DO - 10.1186/s13059-017-1246-7

M3 - Article

C2 - 28619072

SN - 1474-760X

VL - 18

JO - Genome biology

JF - Genome biology

IS - 1

ER -

The Arabidopsis SWI/SNF protein BAF60 mediates seedling growth control by modulating DNA accessibility

Abstract

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