Targeted gene deletions in C. elegans using transposon excision

Christian Frøkjær-Jensen; M. Wayne Davis; Gunther Hollopeter; Jon Taylor; Todd W. Harris; Paola Nix; Rachel Lofgren; Michael Prestgard-Duke; Michael Bastiani; Donald G. Moerman; Erik M. Jorgensen

doi:10.1038/nmeth.1454

Targeted gene deletions in C. elegans using transposon excision

Christian Frøkjær-Jensen, M. Wayne Davis, Gunther Hollopeter, Jon Taylor, Todd W. Harris, Paola Nix, Rachel Lofgren, Michael Prestgard-Duke, Michael Bastiani, Donald G. Moerman, Erik M. Jorgensen

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.

Original language	English (US)
Pages (from-to)	451-453
Number of pages	3
Journal	Nature Methods
Volume	7
Issue number	6
DOIs	https://doi.org/10.1038/nmeth.1454
State	Published - Jun 2010
Externally published	Yes

Bibliographical note

Funding Information:
We thank L. Segalat (Centre National de la Recherche Scientifique, Lyon, France) and members of the NemaGENETAG consortium for Mos1 strains. C.F.-J. is funded by a fellowship from the Lundbeck Foundation and G.H. by a Jane Coffin Childs fellowship funded by Howard Hughes Medical Institute. M.B. is funded by a McKnight Grant. D.G.M. is funded by Genome Canada and Genome British Columbia.

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1038/nmeth.1454

Cite this

@article{fdd41c78472c4c888b5b672781d441f2,

title = "Targeted gene deletions in C. elegans using transposon excision",

abstract = "We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.",

author = "Christian Fr{\o}kj{\ae}r-Jensen and Davis, {M. Wayne} and Gunther Hollopeter and Jon Taylor and Harris, {Todd W.} and Paola Nix and Rachel Lofgren and Michael Prestgard-Duke and Michael Bastiani and Moerman, {Donald G.} and Jorgensen, {Erik M.}",

note = "Funding Information: We thank L. Segalat (Centre National de la Recherche Scientifique, Lyon, France) and members of the NemaGENETAG consortium for Mos1 strains. C.F.-J. is funded by a fellowship from the Lundbeck Foundation and G.H. by a Jane Coffin Childs fellowship funded by Howard Hughes Medical Institute. M.B. is funded by a McKnight Grant. D.G.M. is funded by Genome Canada and Genome British Columbia.",

year = "2010",

month = jun,

doi = "10.1038/nmeth.1454",

language = "English (US)",

volume = "7",

pages = "451--453",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Publishing Group",

number = "6",

}

TY - JOUR

T1 - Targeted gene deletions in C. elegans using transposon excision

AU - Frøkjær-Jensen, Christian

AU - Davis, M. Wayne

AU - Hollopeter, Gunther

AU - Taylor, Jon

AU - Harris, Todd W.

AU - Nix, Paola

AU - Lofgren, Rachel

AU - Prestgard-Duke, Michael

AU - Bastiani, Michael

AU - Moerman, Donald G.

AU - Jorgensen, Erik M.

N1 - Funding Information: We thank L. Segalat (Centre National de la Recherche Scientifique, Lyon, France) and members of the NemaGENETAG consortium for Mos1 strains. C.F.-J. is funded by a fellowship from the Lundbeck Foundation and G.H. by a Jane Coffin Childs fellowship funded by Howard Hughes Medical Institute. M.B. is funded by a McKnight Grant. D.G.M. is funded by Genome Canada and Genome British Columbia.

PY - 2010/6

Y1 - 2010/6

N2 - We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.

AB - We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.

UR - http://www.scopus.com/inward/record.url?scp=77952993846&partnerID=8YFLogxK

U2 - 10.1038/nmeth.1454

DO - 10.1038/nmeth.1454

M3 - Article

C2 - 20418868

AN - SCOPUS:77952993846

SN - 1548-7091

VL - 7

SP - 451

EP - 453

JO - Nature Methods

JF - Nature Methods

IS - 6

ER -

Targeted gene deletions in C. elegans using transposon excision

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this