Tailoring the Surface Chemistry of PEDOT:PSS to Promote Supported Lipid Bilayer Formation

Konstantinos Kallitsis; Anna-Maria Pappa; Zixuan Lu; Alberto Alvarez-Fernandez; Ioanna Charalambous; Sina Schack; Walther C. Traberg; Quentin Thiburce; Karan Bali; Graham Christie; Stefan Guldin; Susan Daniel; Alberto Salleo; R. M. Owens

doi:10.1002/mame.202300038

Tailoring the Surface Chemistry of PEDOT:PSS to Promote Supported Lipid Bilayer Formation

Konstantinos Kallitsis, Anna-Maria Pappa, Zixuan Lu, Alberto Alvarez-Fernandez, Ioanna Charalambous, Sina Schack, Walther C. Traberg, Quentin Thiburce, Karan Bali, Graham Christie, Stefan Guldin, Susan Daniel, Alberto Salleo, R. M. Owens

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

This communication reports on a versatile and substrate-agnostic method to tune the surface chemistry of conducting polymers with the aim of bridging the chemical mismatch between bioelectronic devices and biological systems. As a proof of concept, the surface of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) is grafted with a short-chain oligoethylene glycol monolayer to favor the formation of cell-derived supported lipid bilayers (SLBs). This method is tuned to optimize the affinity between the supported lipid bilayer and the conducting polymer, leading to significant improvements in bilayer quality and therefore electronic readouts. To validate the impact of surface functionalization on the system's ability to transduce biological phenomena into quantifiable electronic signals, the activity of a virus commonly used as a surrogate for SARS-CoV-2 (mouse hepatitis virus) is monitored with and without surface treatment. The functionalized devices exhibit significant improvements in electronic output, stemming from the improved SLB quality, therefore strengthening the case for the use of such an approach in membrane-on-a-chip systems.

Original language	English (US)
Journal	Macromolecular Materials and Engineering
DOIs	https://doi.org/10.1002/mame.202300038
State	Published - Apr 14 2023
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2023-05-09
Acknowledged KAUST grant number(s): OSR-2018-CRG7-3709
Acknowledgements: The authors acknowledge funding for this project, sponsored by the Defense Advanced Research Projects Agency (DARPA) Army Research Office and accomplished under Cooperative Agreement Number W911NF-18-2-0152. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of DARPA or the Army Research Office or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. This publication was also supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-2018-CRG7-3709. A.A.F. and S.G. are grateful for funding by an EPSRC New Investigator Award (EP/R035105/1). WT acknowledges funding from the Cambridge Trust. Part of this work was performed at the Stanford Nanofabrication Facilities (SNF) and Stanford Nano Shared Facilities (SNSF), supported by the National Science Foundation as part of the National Nanotechnology Coordinated Infrastructure under award ECCS-1542152. The schematic illustrations on Figures 2 and 3 were created using Biorender.com. Finally, the authors acknowledge Dr. Achilleas Savva for fruitful discussions regarding the virus fusion experiments.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

ASJC Scopus subject areas

Materials Chemistry
Organic Chemistry
Polymers and Plastics
General Chemical Engineering

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/mame.202300038

Cite this

Kallitsis, K., Pappa, A.-M., Lu, Z., Alvarez-Fernandez, A., Charalambous, I., Schack, S., Traberg, W. C., Thiburce, Q., Bali, K., Christie, G., Guldin, S., Daniel, S., Salleo, A., & Owens, R. M. (2023). Tailoring the Surface Chemistry of PEDOT:PSS to Promote Supported Lipid Bilayer Formation. Macromolecular Materials and Engineering. https://doi.org/10.1002/mame.202300038

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title = "Tailoring the Surface Chemistry of PEDOT:PSS to Promote Supported Lipid Bilayer Formation",

abstract = "This communication reports on a versatile and substrate-agnostic method to tune the surface chemistry of conducting polymers with the aim of bridging the chemical mismatch between bioelectronic devices and biological systems. As a proof of concept, the surface of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) is grafted with a short-chain oligoethylene glycol monolayer to favor the formation of cell-derived supported lipid bilayers (SLBs). This method is tuned to optimize the affinity between the supported lipid bilayer and the conducting polymer, leading to significant improvements in bilayer quality and therefore electronic readouts. To validate the impact of surface functionalization on the system's ability to transduce biological phenomena into quantifiable electronic signals, the activity of a virus commonly used as a surrogate for SARS-CoV-2 (mouse hepatitis virus) is monitored with and without surface treatment. The functionalized devices exhibit significant improvements in electronic output, stemming from the improved SLB quality, therefore strengthening the case for the use of such an approach in membrane-on-a-chip systems.",

author = "Konstantinos Kallitsis and Anna-Maria Pappa and Zixuan Lu and Alberto Alvarez-Fernandez and Ioanna Charalambous and Sina Schack and Traberg, {Walther C.} and Quentin Thiburce and Karan Bali and Graham Christie and Stefan Guldin and Susan Daniel and Alberto Salleo and Owens, {R. M.}",

note = "KAUST Repository Item: Exported on 2023-05-09 Acknowledged KAUST grant number(s): OSR-2018-CRG7-3709 Acknowledgements: The authors acknowledge funding for this project, sponsored by the Defense Advanced Research Projects Agency (DARPA) Army Research Office and accomplished under Cooperative Agreement Number W911NF-18-2-0152. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of DARPA or the Army Research Office or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. This publication was also supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-2018-CRG7-3709. A.A.F. and S.G. are grateful for funding by an EPSRC New Investigator Award (EP/R035105/1). WT acknowledges funding from the Cambridge Trust. Part of this work was performed at the Stanford Nanofabrication Facilities (SNF) and Stanford Nano Shared Facilities (SNSF), supported by the National Science Foundation as part of the National Nanotechnology Coordinated Infrastructure under award ECCS-1542152. The schematic illustrations on Figures 2 and 3 were created using Biorender.com. Finally, the authors acknowledge Dr. Achilleas Savva for fruitful discussions regarding the virus fusion experiments. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

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AU - Alvarez-Fernandez, Alberto

AU - Charalambous, Ioanna

AU - Schack, Sina

AU - Traberg, Walther C.

AU - Thiburce, Quentin

AU - Bali, Karan

AU - Christie, Graham

AU - Guldin, Stefan

AU - Daniel, Susan

AU - Salleo, Alberto

AU - Owens, R. M.

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N2 - This communication reports on a versatile and substrate-agnostic method to tune the surface chemistry of conducting polymers with the aim of bridging the chemical mismatch between bioelectronic devices and biological systems. As a proof of concept, the surface of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) is grafted with a short-chain oligoethylene glycol monolayer to favor the formation of cell-derived supported lipid bilayers (SLBs). This method is tuned to optimize the affinity between the supported lipid bilayer and the conducting polymer, leading to significant improvements in bilayer quality and therefore electronic readouts. To validate the impact of surface functionalization on the system's ability to transduce biological phenomena into quantifiable electronic signals, the activity of a virus commonly used as a surrogate for SARS-CoV-2 (mouse hepatitis virus) is monitored with and without surface treatment. The functionalized devices exhibit significant improvements in electronic output, stemming from the improved SLB quality, therefore strengthening the case for the use of such an approach in membrane-on-a-chip systems.

AB - This communication reports on a versatile and substrate-agnostic method to tune the surface chemistry of conducting polymers with the aim of bridging the chemical mismatch between bioelectronic devices and biological systems. As a proof of concept, the surface of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) is grafted with a short-chain oligoethylene glycol monolayer to favor the formation of cell-derived supported lipid bilayers (SLBs). This method is tuned to optimize the affinity between the supported lipid bilayer and the conducting polymer, leading to significant improvements in bilayer quality and therefore electronic readouts. To validate the impact of surface functionalization on the system's ability to transduce biological phenomena into quantifiable electronic signals, the activity of a virus commonly used as a surrogate for SARS-CoV-2 (mouse hepatitis virus) is monitored with and without surface treatment. The functionalized devices exhibit significant improvements in electronic output, stemming from the improved SLB quality, therefore strengthening the case for the use of such an approach in membrane-on-a-chip systems.

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Tailoring the Surface Chemistry of PEDOT:PSS to Promote Supported Lipid Bilayer Formation

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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