TY - JOUR
T1 - Synthesis of robust electrochemical substrate and fabrication of immobilization free biosensors for rapid sensing of salicylate and β-hydroxybutyrate in whole blood
AU - Chen, Ting Yo
AU - Mani, Veerappan
AU - Huang, Sheng Tung
AU - Chang, Pu Chieh
AU - Huang, Chih Hung
AU - Huang, Nay Ming
N1 - Generated from Scopus record by KAUST IRTS on 2023-09-21
PY - 2017/10/16
Y1 - 2017/10/16
N2 - An electrochemical latent redox probe, SAF 5 was designed, synthesized and characterized. A rapid and sensitive solution-based assay was demonstrated for salicylate hydroxylase (SHL). In presence of NADH at aerobic conditions, SHL catalyzed the decarboxylative hydroxylation of SAF and released a redox reporter amino ferrocene (AF 6). The release of AF 6 was monitored at interference free potential region (–50 mV vs. Ag|AgCl) using differential pulse voltammetry as signal read-out. The current signal generated by this process is highly specific, and insensitive to other biological interfering compounds. Next, the SAF incorporated SHL assay was extended to fabricate immobilization-free biosensors for rapid sensing of salicylic acid (SA) and β-hydroxybutyrate (β-HB) in whole blood. The described method rapidly detects SA in a linear range of 35–560 μM with detection limit of 5.0 μM. For β-HB determination, the linear range was 10–600 μM and detection limit was 2.0 μM. Besides, the assay protocols are simple, fast, reliable, selective, sensitive and advantageous over existing methods. The whole blood assay did not required cumbersome steps such as, enzyme immobilization, pre-treatments and holds great practical potential in clinical diagnosis.
AB - An electrochemical latent redox probe, SAF 5 was designed, synthesized and characterized. A rapid and sensitive solution-based assay was demonstrated for salicylate hydroxylase (SHL). In presence of NADH at aerobic conditions, SHL catalyzed the decarboxylative hydroxylation of SAF and released a redox reporter amino ferrocene (AF 6). The release of AF 6 was monitored at interference free potential region (–50 mV vs. Ag|AgCl) using differential pulse voltammetry as signal read-out. The current signal generated by this process is highly specific, and insensitive to other biological interfering compounds. Next, the SAF incorporated SHL assay was extended to fabricate immobilization-free biosensors for rapid sensing of salicylic acid (SA) and β-hydroxybutyrate (β-HB) in whole blood. The described method rapidly detects SA in a linear range of 35–560 μM with detection limit of 5.0 μM. For β-HB determination, the linear range was 10–600 μM and detection limit was 2.0 μM. Besides, the assay protocols are simple, fast, reliable, selective, sensitive and advantageous over existing methods. The whole blood assay did not required cumbersome steps such as, enzyme immobilization, pre-treatments and holds great practical potential in clinical diagnosis.
UR - https://linkinghub.elsevier.com/retrieve/pii/S0003267017310656
UR - http://www.scopus.com/inward/record.url?scp=85029490374&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2017.08.051
DO - 10.1016/j.aca.2017.08.051
M3 - Article
SN - 0003-2670
VL - 990
SP - 78
EP - 83
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -