Superhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour

N. Malara; F. Gentile; N. Coppedè; M. L. Coluccio; P. Candeloro; G. Perozziello; L. Ferrara; M. Giannetto; M. Careri; A. Castellini; C. Mignogna; I. Presta; C. K. Pirrone; D. Maisano; A. Donato; G. Donato; M. Greco; D. Scumaci; Giovanni Cuda; F. Casale; E. Ferraro; S. Bonacci; V. Trunzo; V. Mollace; V. Onesto; R. Majewska; F. Amato; M. Renne; N. Innaro; G. Sena; R. Sacco; F. Givigliano; C. Voci; G. Volpentesta; G. Guzzi; A. Lavano; E. Scali; U. Bottoni; Enzo M. Di Fabrizio

doi:10.1038/s41698-018-0069-7

Superhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour

N. Malara, F. Gentile, N. Coppedè, M. L. Coluccio, P. Candeloro, G. Perozziello, L. Ferrara, M. Giannetto, M. Careri, A. Castellini, C. Mignogna, I. Presta, C. K. Pirrone, D. Maisano, A. Donato, G. Donato, M. Greco, D. Scumaci, Giovanni Cuda, F. CasaleE. Ferraro, S. Bonacci, V. Trunzo, V. Mollace, V. Onesto, R. Majewska, F. Amato, M. Renne, N. Innaro, G. Sena, R. Sacco, F. Givigliano, C. Voci, G. Volpentesta, G. Guzzi, A. Lavano, E. Scali, U. Bottoni, Enzo M. Di Fabrizio

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23 Scopus citations

Abstract

Secretome of primary cultures is an accessible source of biological markers compared to more complex and less decipherable mixtures such as serum or plasma. The protonation state (PS) of secretome reflects the metabolism of cells and can be used for cancer early detection. Here, we demonstrate a superhydrophobic organic electrochemical device that measures PS in a drop of secretome derived from liquid biopsies. Using data from the sensor and principal component analysis (PCA), we developed algorithms able to efficiently discriminate tumour patients from non-tumour patients. We then validated the results using mass spectrometry and biochemical analysis of samples. For the 36 patients across three independent cohorts, the method identified tumour patients with high sensitivity and identification as high as 100% (no false positives) with declared subjects at-risk, for sporadic cancer onset, by intermediate values of PS. This assay could impact on cancer risk management, individual's diagnosis and/or help clarify risk in healthy populations.

Original language	English (US)
Journal	npj Precision Oncology
Volume	2
Issue number	1
DOIs	https://doi.org/10.1038/s41698-018-0069-7
State	Published - Nov 19 2018

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): OCRF-2016-CRG5
Acknowledgements: This paper is dedicated to the memory of our colleague Prof. Ugo Bottoni. This work has been partially funded from the Italian Ministry of Health (Project n. GR-2010–2320665) and (Project n. GR-2010–2311677) by the project Interregional Research Centre for Food Safety & Health (IRC_FSH) (cod. PON a3–00359), granted to the Department of Health Science, University of Magna Graecia. The authors also acknowledge financial support from KAUST funding through CRG5 project OSR award #: OCRF-2016-CRG5. NC acknowledges Nicola Zambelli and Giacomo Benassi for technical support. NM acknowledges Prof. Bruno Silvestrini and Gianluca Rotta for their precious scientific support and Nastassia C. Garo for her technical assistance. The authors would like to thank the patients and all health professionals involved at the University of 'Magna Graecia' and Mater Domini Hospital and ANASTE nursing home.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41698-018-0069-7

https://repository.kaust.edu.sa/bitstream/10754/630183/1/s41698-018-0069-7.pdf

Cite this

Malara, N., Gentile, F., Coppedè, N., Coluccio, M. L., Candeloro, P., Perozziello, G., Ferrara, L., Giannetto, M., Careri, M., Castellini, A., Mignogna, C., Presta, I., Pirrone, C. K., Maisano, D., Donato, A., Donato, G., Greco, M., Scumaci, D., Cuda, G., ... Di Fabrizio, E. M. (2018). Superhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour. npj Precision Oncology, 2(1). https://doi.org/10.1038/s41698-018-0069-7

@article{b481ac01022744588df5c1534c823210,

title = "Superhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour",

abstract = "Secretome of primary cultures is an accessible source of biological markers compared to more complex and less decipherable mixtures such as serum or plasma. The protonation state (PS) of secretome reflects the metabolism of cells and can be used for cancer early detection. Here, we demonstrate a superhydrophobic organic electrochemical device that measures PS in a drop of secretome derived from liquid biopsies. Using data from the sensor and principal component analysis (PCA), we developed algorithms able to efficiently discriminate tumour patients from non-tumour patients. We then validated the results using mass spectrometry and biochemical analysis of samples. For the 36 patients across three independent cohorts, the method identified tumour patients with high sensitivity and identification as high as 100% (no false positives) with declared subjects at-risk, for sporadic cancer onset, by intermediate values of PS. This assay could impact on cancer risk management, individual's diagnosis and/or help clarify risk in healthy populations.",

author = "N. Malara and F. Gentile and N. Copped{\`e} and Coluccio, {M. L.} and P. Candeloro and G. Perozziello and L. Ferrara and M. Giannetto and M. Careri and A. Castellini and C. Mignogna and I. Presta and Pirrone, {C. K.} and D. Maisano and A. Donato and G. Donato and M. Greco and D. Scumaci and Giovanni Cuda and F. Casale and E. Ferraro and S. Bonacci and V. Trunzo and V. Mollace and V. Onesto and R. Majewska and F. Amato and M. Renne and N. Innaro and G. Sena and R. Sacco and F. Givigliano and C. Voci and G. Volpentesta and G. Guzzi and A. Lavano and E. Scali and U. Bottoni and {Di Fabrizio}, {Enzo M.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): OCRF-2016-CRG5 Acknowledgements: This paper is dedicated to the memory of our colleague Prof. Ugo Bottoni. This work has been partially funded from the Italian Ministry of Health (Project n. GR-2010–2320665) and (Project n. GR-2010–2311677) by the project Interregional Research Centre for Food Safety & Health (IRC_FSH) (cod. PON a3–00359), granted to the Department of Health Science, University of Magna Graecia. The authors also acknowledge financial support from KAUST funding through CRG5 project OSR award #: OCRF-2016-CRG5. NC acknowledges Nicola Zambelli and Giacomo Benassi for technical support. NM acknowledges Prof. Bruno Silvestrini and Gianluca Rotta for their precious scientific support and Nastassia C. Garo for her technical assistance. The authors would like to thank the patients and all health professionals involved at the University of 'Magna Graecia' and Mater Domini Hospital and ANASTE nursing home.",

year = "2018",

month = nov,

day = "19",

doi = "10.1038/s41698-018-0069-7",

language = "English (US)",

volume = "2",

journal = "npj Precision Oncology",

issn = "2397-768X",

publisher = "Springer Nature",

number = "1",

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Malara, N, Gentile, F, Coppedè, N, Coluccio, ML, Candeloro, P, Perozziello, G, Ferrara, L, Giannetto, M, Careri, M, Castellini, A, Mignogna, C, Presta, I, Pirrone, CK, Maisano, D, Donato, A, Donato, G, Greco, M, Scumaci, D, Cuda, G, Casale, F, Ferraro, E, Bonacci, S, Trunzo, V, Mollace, V, Onesto, V, Majewska, R, Amato, F, Renne, M, Innaro, N, Sena, G, Sacco, R, Givigliano, F, Voci, C, Volpentesta, G, Guzzi, G, Lavano, A, Scali, E, Bottoni, U & Di Fabrizio, EM 2018, 'Superhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour', npj Precision Oncology, vol. 2, no. 1. https://doi.org/10.1038/s41698-018-0069-7

TY - JOUR

T1 - Superhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour

AU - Malara, N.

AU - Gentile, F.

AU - Coppedè, N.

AU - Coluccio, M. L.

AU - Candeloro, P.

AU - Perozziello, G.

AU - Ferrara, L.

AU - Giannetto, M.

AU - Careri, M.

AU - Castellini, A.

AU - Mignogna, C.

AU - Presta, I.

AU - Pirrone, C. K.

AU - Maisano, D.

AU - Donato, A.

AU - Donato, G.

AU - Greco, M.

AU - Scumaci, D.

AU - Cuda, Giovanni

AU - Casale, F.

AU - Ferraro, E.

AU - Bonacci, S.

AU - Trunzo, V.

AU - Mollace, V.

AU - Onesto, V.

AU - Majewska, R.

AU - Amato, F.

AU - Renne, M.

AU - Innaro, N.

AU - Sena, G.

AU - Sacco, R.

AU - Givigliano, F.

AU - Voci, C.

AU - Volpentesta, G.

AU - Guzzi, G.

AU - Lavano, A.

AU - Scali, E.

AU - Bottoni, U.

AU - Di Fabrizio, Enzo M.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): OCRF-2016-CRG5 Acknowledgements: This paper is dedicated to the memory of our colleague Prof. Ugo Bottoni. This work has been partially funded from the Italian Ministry of Health (Project n. GR-2010–2320665) and (Project n. GR-2010–2311677) by the project Interregional Research Centre for Food Safety & Health (IRC_FSH) (cod. PON a3–00359), granted to the Department of Health Science, University of Magna Graecia. The authors also acknowledge financial support from KAUST funding through CRG5 project OSR award #: OCRF-2016-CRG5. NC acknowledges Nicola Zambelli and Giacomo Benassi for technical support. NM acknowledges Prof. Bruno Silvestrini and Gianluca Rotta for their precious scientific support and Nastassia C. Garo for her technical assistance. The authors would like to thank the patients and all health professionals involved at the University of 'Magna Graecia' and Mater Domini Hospital and ANASTE nursing home.

PY - 2018/11/19

Y1 - 2018/11/19

N2 - Secretome of primary cultures is an accessible source of biological markers compared to more complex and less decipherable mixtures such as serum or plasma. The protonation state (PS) of secretome reflects the metabolism of cells and can be used for cancer early detection. Here, we demonstrate a superhydrophobic organic electrochemical device that measures PS in a drop of secretome derived from liquid biopsies. Using data from the sensor and principal component analysis (PCA), we developed algorithms able to efficiently discriminate tumour patients from non-tumour patients. We then validated the results using mass spectrometry and biochemical analysis of samples. For the 36 patients across three independent cohorts, the method identified tumour patients with high sensitivity and identification as high as 100% (no false positives) with declared subjects at-risk, for sporadic cancer onset, by intermediate values of PS. This assay could impact on cancer risk management, individual's diagnosis and/or help clarify risk in healthy populations.

AB - Secretome of primary cultures is an accessible source of biological markers compared to more complex and less decipherable mixtures such as serum or plasma. The protonation state (PS) of secretome reflects the metabolism of cells and can be used for cancer early detection. Here, we demonstrate a superhydrophobic organic electrochemical device that measures PS in a drop of secretome derived from liquid biopsies. Using data from the sensor and principal component analysis (PCA), we developed algorithms able to efficiently discriminate tumour patients from non-tumour patients. We then validated the results using mass spectrometry and biochemical analysis of samples. For the 36 patients across three independent cohorts, the method identified tumour patients with high sensitivity and identification as high as 100% (no false positives) with declared subjects at-risk, for sporadic cancer onset, by intermediate values of PS. This assay could impact on cancer risk management, individual's diagnosis and/or help clarify risk in healthy populations.

UR - http://hdl.handle.net/10754/630183

UR - https://www.nature.com/articles/s41698-018-0069-7

U2 - 10.1038/s41698-018-0069-7

DO - 10.1038/s41698-018-0069-7

M3 - Article

C2 - 30480095

SN - 2397-768X

VL - 2

JO - npj Precision Oncology

JF - npj Precision Oncology

IS - 1

ER -

Superhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour

Abstract

Bibliographical note

UN SDGs

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