Sulphate-reducing bacteria (SRB) cause fouling, souring, corrosion and produce H2S during oil and gas production. Produced water obtained from Periquito (PQO) and Galo de Campina (GC) onshore oilfields in Brazil was investigated for SRB. Produced water with Postgate B, Postgate C and Baars media was incubated anaerobically for 20 days. DNA was extracted, 16S rDNA PCR amplified and fragments were sequenced using Illumina TruSeq. 4.2 million sequence reads were analysed and deposited at NCBI SAR accession number SRP149784. No significant differences in microbial community composition could be attributed to the different media but significant differences in the SRB were observed between the two oil fields. The dominant bacterial orders detected from both oilfields were Desulfovibrionales, Pseudomonadales and Enterobacteriales. The genus Pseudomonas was found predominantly in the GC oilfield and Pleomorphominas and Shewanella were features of the PQO oilfield. 11% and 7.6% of the sequences at GC and PQO were not classified at the genus level but could be partially identified at the order level. Relative abundances changed for Desulfovibrio from 29.8% at PQO to 16.1% at GC. Clostridium varied from 2.8% at PQO and 2.4% at GC. These data provide the first description of SRB from onshore produced water in Brazil and reinforce the importance of Desulfovibrionales, Pseudomonadales, and Enterobacteriales in produced water globally. Identifying potentially harmful microbes is an important first step in developing microbial solutions that prevent their proliferation.
KAUST Repository Item: Exported on 2021-10-19
Acknowledgements: This work was supported in part by the Coordenação de Aperfeiçoamento Pessoal de Nível Superior-Brasil (CAPES), Finance code 001, by grants from Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, grant code “CIENTISTA DO NOSSO ESTADO” 26/202. 630/2019 ), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (MCTI-CNPq, grant code 309461/2019–7). SRGT thanks the Postgraduate Programme in Plant Biotechnology and Bioprocesses at Universidade Federal do Rio de Janeiro (UFRJ) and Coordenação de Aperfeiçoamento Pessoal de Nível Superior-Brasil (CAPES) for her PhD grant funding.