Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Jonas Kühn*, Frédéric Montfort, Tristan Colomb, Benjamin Rappaz, Corinne Moratal, Nicolas Pavillon, Pierre Marquet, Christian Depeursinge

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

77 Scopus citations


We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 μm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.

Original languageEnglish (US)
Pages (from-to)653-655
Number of pages3
JournalOptics Letters
Issue number5
StatePublished - Mar 1 2009

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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