Structure-property relationship in cytotoxicity and cell uptake of poly(2-oxazoline) amphiphiles

Robert Luxenhofer; Gaurav Sahay; Anita Schulz; Daria Alakhova; Tatiana K. Bronich; Rainer Jordan; Alexander V. Kabanov

doi:10.1016/j.jconrel.2011.04.010

Structure-property relationship in cytotoxicity and cell uptake of poly(2-oxazoline) amphiphiles

Robert Luxenhofer, Gaurav Sahay, Anita Schulz, Daria Alakhova, Tatiana K. Bronich, Rainer Jordan, Alexander V. Kabanov

Research output: Contribution to journal › Article › peer-review

178 Scopus citations

Abstract

The family of poly(2-oxazoline)s (POx) is being increasingly investigated in the context of biomedical applications. We tested the relative cytotoxicity of POx and were able to confirm that these polymers are typically not cytotoxic even at high concentrations. Furthermore, we report structure-uptake relationships of a series of amphiphilic POx block copolymers that have different architectures, molar mass and chain termini. The rate of endocytosis can be fine-tuned over a broad range by changing the polymer structure. The cellular uptake increases with the hydrophobic character of the polymers and is observed even at nanomolar concentrations. Considering the structural versatility of this class of polymers, the relative ease of preparation and their stability underlines the potential of POx as a promising platform candidate for the preparation of next-generation polymer therapeutics.

Original language	English (US)
Pages (from-to)	73-82
Number of pages	10
Journal	Journal of Controlled Release
Volume	153
Issue number	1
DOIs	https://doi.org/10.1016/j.jconrel.2011.04.010
State	Published - Jul 2011
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): KUK-F1-029-32
Acknowledgements: RI gratefully acknowledges a postdoctoral fellowship of the Deutscher Akademischer Austauschdienst (DAAD) and the King Abdullah University of Science and Technology (KAUST, Award No. KUK-F1-029-32) for partial salary support. This study was supported by National Institutes of Health grants 1P20 RR021937, UO1 CA151806 and 2RO1 CA89225 awarded to AVK and the DFG Forschergruppe FOR411 "Radionuklidtherapie" awarded to RJ (project P12). We would also like to thank the flow cytometry and confocal microscopy core facilities at UNMC.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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10.1016/j.jconrel.2011.04.010

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title = "Structure-property relationship in cytotoxicity and cell uptake of poly(2-oxazoline) amphiphiles",

abstract = "The family of poly(2-oxazoline)s (POx) is being increasingly investigated in the context of biomedical applications. We tested the relative cytotoxicity of POx and were able to confirm that these polymers are typically not cytotoxic even at high concentrations. Furthermore, we report structure-uptake relationships of a series of amphiphilic POx block copolymers that have different architectures, molar mass and chain termini. The rate of endocytosis can be fine-tuned over a broad range by changing the polymer structure. The cellular uptake increases with the hydrophobic character of the polymers and is observed even at nanomolar concentrations. Considering the structural versatility of this class of polymers, the relative ease of preparation and their stability underlines the potential of POx as a promising platform candidate for the preparation of next-generation polymer therapeutics.",

author = "Robert Luxenhofer and Gaurav Sahay and Anita Schulz and Daria Alakhova and Bronich, {Tatiana K.} and Rainer Jordan and Kabanov, {Alexander V.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): KUK-F1-029-32 Acknowledgements: RI gratefully acknowledges a postdoctoral fellowship of the Deutscher Akademischer Austauschdienst (DAAD) and the King Abdullah University of Science and Technology (KAUST, Award No. KUK-F1-029-32) for partial salary support. This study was supported by National Institutes of Health grants 1P20 RR021937, UO1 CA151806 and 2RO1 CA89225 awarded to AVK and the DFG Forschergruppe FOR411 {"}Radionuklidtherapie{"} awarded to RJ (project P12). We would also like to thank the flow cytometry and confocal microscopy core facilities at UNMC. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

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AU - Jordan, Rainer

AU - Kabanov, Alexander V.

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AB - The family of poly(2-oxazoline)s (POx) is being increasingly investigated in the context of biomedical applications. We tested the relative cytotoxicity of POx and were able to confirm that these polymers are typically not cytotoxic even at high concentrations. Furthermore, we report structure-uptake relationships of a series of amphiphilic POx block copolymers that have different architectures, molar mass and chain termini. The rate of endocytosis can be fine-tuned over a broad range by changing the polymer structure. The cellular uptake increases with the hydrophobic character of the polymers and is observed even at nanomolar concentrations. Considering the structural versatility of this class of polymers, the relative ease of preparation and their stability underlines the potential of POx as a promising platform candidate for the preparation of next-generation polymer therapeutics.

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Structure-property relationship in cytotoxicity and cell uptake of poly(2-oxazoline) amphiphiles

Abstract

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