Structure and Functional Capacity of a Benzene-mineralizing, Nitrate-reducing Microbial Community

Samuel C. Eziuzor, Felipe Borim Corrêa, Shuchan Peng, Junia Schultz, Sabine Kleinsteuber, Ulisses Nunes da Rocha, Lorenz Adrian, Carsten Vogt

Research output: Contribution to journalArticlepeer-review

Abstract

AimsHow benzene is metabolized by microbes under anoxic conditions is not fully understood. Here, we studied the degradation pathways in a benzene-mineralizing, nitrate-reducing enrichment culture.Methods and resultsBenzene mineralization was dependent on the presence of nitrate and correlated to enrichment of a Peptococcaceae phylotype only distantly related to known anaerobic benzene degraders of this family. Its relative abundance decreased after benzene mineralization had terminated, while other abundant taxa - Ignavibacteriaceae, Rhodanobacteraceae and Brocadiaceae - slightly increased. Generally, the microbial community remained diverse despite amendment of benzene as single organic carbon source, suggesting complex trophic interactions between different functional groups. A subunit of the putative anaerobic benzene carboxylase (AbcA) previously detected in Peptococcaceae was identified by metaproteomic analysis suggesting that benzene was activated by carboxylation. Detection of proteins involved in anaerobic ammonium oxidation (anammox) indicates that benzene mineralization was accompanied by anammox, facilitated by nitrite accumulation and the presence of ammonium in the growth medium.ConclusionsThe results suggest that benzene was activated by carboxylation and further assimilated by a novel Peptococcaceae phylotype.Significance and impact of the studyThe results confirm the hypothesis that Peptococcaceae are important anaerobic benzene degraders.
Original languageEnglish (US)
JournalJournal of Applied Microbiology
DOIs
StatePublished - Jan 7 2022

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

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