StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity

S. L. Samodelov; H. M. Beyer; X. Guo; M. Augustin; K.-P. Jia; Lina Baz; O. Ebenho h; P. Beyer; W. Weber; Salim Al-Babili; M. D. Zurbriggen

doi:10.1126/sciadv.1601266

StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity

S. L. Samodelov, H. M. Beyer, X. Guo, M. Augustin, K.-P. Jia, Lina Baz, O. Ebenho h, P. Beyer, W. Weber, Salim Al-Babili, M. D. Zurbriggen

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47 Scopus citations

Abstract

Strigolactones are key regulators of plant development and interaction with symbiotic fungi; however, quantitative tools for strigolactone signaling analysis are lacking. We introduce a genetically encoded hormone biosensor used to analyze strigolactone-mediated processes, including the study of the components involved in the hormone perception/signaling complex and the structural specificity and sensitivity of natural and synthetic strigolactones in Arabidopsis, providing quantitative insights into the stereoselectivity of strigolactone perception. Given the high specificity, sensitivity, dynamic range of activity, modular construction, ease of implementation, and wide applicability, the biosensor StrigoQuant will be useful in unraveling multiple levels of strigolactone metabolic and signaling networks.

Original language	English (US)
Pages (from-to)	e1601266
Journal	Science advances
Volume	2
Issue number	11
DOIs	https://doi.org/10.1126/sciadv.1601266
State	Published - Nov 4 2016

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This research was supported by funding from the excellence initiatives of the German federal and state governments (DFG EXC-1028-CEPLAS, EXC 294-BIOSS, and GSC 4-SGBM) and the King Abdullah University of Science and Technology (KAUST).

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title = "StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity",

abstract = "Strigolactones are key regulators of plant development and interaction with symbiotic fungi; however, quantitative tools for strigolactone signaling analysis are lacking. We introduce a genetically encoded hormone biosensor used to analyze strigolactone-mediated processes, including the study of the components involved in the hormone perception/signaling complex and the structural specificity and sensitivity of natural and synthetic strigolactones in Arabidopsis, providing quantitative insights into the stereoselectivity of strigolactone perception. Given the high specificity, sensitivity, dynamic range of activity, modular construction, ease of implementation, and wide applicability, the biosensor StrigoQuant will be useful in unraveling multiple levels of strigolactone metabolic and signaling networks.",

author = "Samodelov, {S. L.} and Beyer, {H. M.} and X. Guo and M. Augustin and K.-P. Jia and Lina Baz and O. Ebenho h and P. Beyer and W. Weber and Salim Al-Babili and Zurbriggen, {M. D.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This research was supported by funding from the excellence initiatives of the German federal and state governments (DFG EXC-1028-CEPLAS, EXC 294-BIOSS, and GSC 4-SGBM) and the King Abdullah University of Science and Technology (KAUST).",

year = "2016",

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doi = "10.1126/sciadv.1601266",

language = "English (US)",

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T1 - StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity

AU - Samodelov, S. L.

AU - Beyer, H. M.

AU - Guo, X.

AU - Augustin, M.

AU - Jia, K.-P.

AU - Baz, Lina

AU - Ebenho h, O.

AU - Beyer, P.

AU - Weber, W.

AU - Al-Babili, Salim

AU - Zurbriggen, M. D.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This research was supported by funding from the excellence initiatives of the German federal and state governments (DFG EXC-1028-CEPLAS, EXC 294-BIOSS, and GSC 4-SGBM) and the King Abdullah University of Science and Technology (KAUST).

PY - 2016/11/4

Y1 - 2016/11/4

N2 - Strigolactones are key regulators of plant development and interaction with symbiotic fungi; however, quantitative tools for strigolactone signaling analysis are lacking. We introduce a genetically encoded hormone biosensor used to analyze strigolactone-mediated processes, including the study of the components involved in the hormone perception/signaling complex and the structural specificity and sensitivity of natural and synthetic strigolactones in Arabidopsis, providing quantitative insights into the stereoselectivity of strigolactone perception. Given the high specificity, sensitivity, dynamic range of activity, modular construction, ease of implementation, and wide applicability, the biosensor StrigoQuant will be useful in unraveling multiple levels of strigolactone metabolic and signaling networks.

AB - Strigolactones are key regulators of plant development and interaction with symbiotic fungi; however, quantitative tools for strigolactone signaling analysis are lacking. We introduce a genetically encoded hormone biosensor used to analyze strigolactone-mediated processes, including the study of the components involved in the hormone perception/signaling complex and the structural specificity and sensitivity of natural and synthetic strigolactones in Arabidopsis, providing quantitative insights into the stereoselectivity of strigolactone perception. Given the high specificity, sensitivity, dynamic range of activity, modular construction, ease of implementation, and wide applicability, the biosensor StrigoQuant will be useful in unraveling multiple levels of strigolactone metabolic and signaling networks.

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SP - e1601266

JO - Science advances

JF - Science advances

IS - 11

ER -

StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity

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