Stem rust resistance in wheat is suppressed by a subunit of the mediator complex

Colin W. Hiebert; Matthew J. Moscou; Tim Hewitt; Burkhard Steuernagel; Inma Hernández-Pinzón; Phon Green; Vincent Pujol; Peng Zhang; Matthew N. Rouse; Yue Jin; Robert A. McIntosh; Narayana Upadhyaya; Jianping Zhang; Sridhar Bhavani; Jan Vrána; Miroslava Karafiátová; Li Huang; Tom Fetch; Jaroslav Doležel; Brande B.H. Wulff; Evans Lagudah; Wolfgang Spielmeyer

doi:10.1038/s41467-020-14937-2

Stem rust resistance in wheat is suppressed by a subunit of the mediator complex

Colin W. Hiebert, Matthew J. Moscou, Tim Hewitt, Burkhard Steuernagel, Inma Hernández-Pinzón, Phon Green, Vincent Pujol, Peng Zhang, Matthew N. Rouse, Yue Jin, Robert A. McIntosh, Narayana Upadhyaya, Jianping Zhang, Sridhar Bhavani, Jan Vrána, Miroslava Karafiátová, Li Huang, Tom Fetch, Jaroslav Doležel, Brande B.H. WulffEvans Lagudah, Wolfgang Spielmeyer

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.

Original language	English (US)
Journal	Nature Communications
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41467-020-14937-2
State	Published - Dec 1 2020
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-20

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Chemistry
General Physics and Astronomy

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10.1038/s41467-020-14937-2

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Hiebert, C. W., Moscou, M. J., Hewitt, T., Steuernagel, B., Hernández-Pinzón, I., Green, P., Pujol, V., Zhang, P., Rouse, M. N., Jin, Y., McIntosh, R. A., Upadhyaya, N., Zhang, J., Bhavani, S., Vrána, J., Karafiátová, M., Huang, L., Fetch, T., Doležel, J., ... Spielmeyer, W. (2020). Stem rust resistance in wheat is suppressed by a subunit of the mediator complex. Nature Communications, 11(1). https://doi.org/10.1038/s41467-020-14937-2

@article{5ee7131c69e44764b6368e44e8a73401,

title = "Stem rust resistance in wheat is suppressed by a subunit of the mediator complex",

abstract = "Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar {\textquoteleft}Canthatch{\textquoteright} suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.",

author = "Hiebert, {Colin W.} and Moscou, {Matthew J.} and Tim Hewitt and Burkhard Steuernagel and Inma Hern{\'a}ndez-Pinz{\'o}n and Phon Green and Vincent Pujol and Peng Zhang and Rouse, {Matthew N.} and Yue Jin and McIntosh, {Robert A.} and Narayana Upadhyaya and Jianping Zhang and Sridhar Bhavani and Jan Vr{\'a}na and Miroslava Karafi{\'a}tov{\'a} and Li Huang and Tom Fetch and Jaroslav Dole{\v z}el and Wulff, {Brande B.H.} and Evans Lagudah and Wolfgang Spielmeyer",

note = "Generated from Scopus record by KAUST IRTS on 2023-02-20",

year = "2020",

month = dec,

day = "1",

doi = "10.1038/s41467-020-14937-2",

language = "English (US)",

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Hiebert, CW, Moscou, MJ, Hewitt, T, Steuernagel, B, Hernández-Pinzón, I, Green, P, Pujol, V, Zhang, P, Rouse, MN, Jin, Y, McIntosh, RA, Upadhyaya, N, Zhang, J, Bhavani, S, Vrána, J, Karafiátová, M, Huang, L, Fetch, T, Doležel, J, Wulff, BBH, Lagudah, E & Spielmeyer, W 2020, 'Stem rust resistance in wheat is suppressed by a subunit of the mediator complex', Nature Communications, vol. 11, no. 1. https://doi.org/10.1038/s41467-020-14937-2

TY - JOUR

T1 - Stem rust resistance in wheat is suppressed by a subunit of the mediator complex

AU - Hiebert, Colin W.

AU - Moscou, Matthew J.

AU - Hewitt, Tim

AU - Steuernagel, Burkhard

AU - Hernández-Pinzón, Inma

AU - Green, Phon

AU - Pujol, Vincent

AU - Zhang, Peng

AU - Rouse, Matthew N.

AU - Jin, Yue

AU - McIntosh, Robert A.

AU - Upadhyaya, Narayana

AU - Zhang, Jianping

AU - Bhavani, Sridhar

AU - Vrána, Jan

AU - Karafiátová, Miroslava

AU - Huang, Li

AU - Fetch, Tom

AU - Doležel, Jaroslav

AU - Wulff, Brande B.H.

AU - Lagudah, Evans

AU - Spielmeyer, Wolfgang

N1 - Generated from Scopus record by KAUST IRTS on 2023-02-20

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.

AB - Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.

UR - https://www.nature.com/articles/s41467-020-14937-2

UR - http://www.scopus.com/inward/record.url?scp=85080992910&partnerID=8YFLogxK

U2 - 10.1038/s41467-020-14937-2

DO - 10.1038/s41467-020-14937-2

M3 - Article

SN - 2041-1723

VL - 11

JO - Nature Communications

JF - Nature Communications

IS - 1

ER -

Stem rust resistance in wheat is suppressed by a subunit of the mediator complex

Abstract

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ASJC Scopus subject areas

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