Standards for plant synthetic biology: A common syntax for exchange of DNA parts

Nicola J. Patron; Diego Orzaez; Sylvestre Marillonnet; Heribert Warzecha; Colette Matthewman; Mark Youles; Oleg Raitskin; Aymeric Leveau; Gemma Farré; Christian Rogers; Alison Smith; Julian Hibberd; Alex A.R. Webb; James Locke; Sebastian Schornack; Jim Ajioka; David C. Baulcombe; Cyril Zipfel; Sophien Kamoun; Jonathan D.G. Jones; Hannah Kuhn; Silke Robatzek; H. Peter Van Esse; Dale Sanders; Giles Oldroyd; Cathie Martin; Rob Field; Sarah O'Connor; Samantha Fox; Brande Wulff; Ben Miller; Andy Breakspear; Guru Radhakrishnan; Pierre Marc Delaux; Dominique Loqué; Antonio Granell; Alain Tissier; Patrick Shih; Thomas P. Brutnell; W. Paul Quick; Heiko Rischer; Paul D. Fraser; Asaph Aharoni; Christine Raines; Paul F. South; Jean Michel Ané; Björn R. Hamberger; Jane Langdale; Jens Stougaard; Harro Bouwmeester; Michael Udvardi; James A.H. Murray; Vardis Ntoukakis; Patrick Schäfer; Katherine Denby; Keith J. Edwards; Anne Osbourn; Jim Haseloff

doi:10.1111/nph.13532

Standards for plant synthetic biology: A common syntax for exchange of DNA parts

Nicola J. Patron, Diego Orzaez, Sylvestre Marillonnet, Heribert Warzecha, Colette Matthewman, Mark Youles, Oleg Raitskin, Aymeric Leveau, Gemma Farré, Christian Rogers, Alison Smith, Julian Hibberd, Alex A.R. Webb, James Locke, Sebastian Schornack, Jim Ajioka, David C. Baulcombe, Cyril Zipfel, Sophien Kamoun, Jonathan D.G. JonesHannah Kuhn, Silke Robatzek, H. Peter Van Esse, Dale Sanders, Giles Oldroyd, Cathie Martin, Rob Field, Sarah O'Connor, Samantha Fox, Brande Wulff, Ben Miller, Andy Breakspear, Guru Radhakrishnan, Pierre Marc Delaux, Dominique Loqué, Antonio Granell, Alain Tissier, Patrick Shih, Thomas P. Brutnell, W. Paul Quick, Heiko Rischer, Paul D. Fraser, Asaph Aharoni, Christine Raines, Paul F. South, Jean Michel Ané, Björn R. Hamberger, Jane Langdale, Jens Stougaard, Harro Bouwmeester, Michael Udvardi, James A.H. Murray, Vardis Ntoukakis, Patrick Schäfer, Katherine Denby, Keith J. Edwards, Anne Osbourn, Jim Haseloff

Research output: Contribution to journal › Article › peer-review

199 Scopus citations

Abstract

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Original language	English (US)
Pages (from-to)	13-19
Number of pages	7
Journal	New Phytologist
Volume	208
Issue number	1
DOIs	https://doi.org/10.1111/nph.13532
State	Published - Oct 1 2015
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-20

ASJC Scopus subject areas

General Medicine

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10.1111/nph.13532

Cite this

Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., Raitskin, O., Leveau, A., Farré, G., Rogers, C., Smith, A., Hibberd, J., Webb, A. A. R., Locke, J., Schornack, S., Ajioka, J., Baulcombe, D. C., Zipfel, C., Kamoun, S., ... Haseloff, J. (2015). Standards for plant synthetic biology: A common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. https://doi.org/10.1111/nph.13532

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title = "Standards for plant synthetic biology: A common syntax for exchange of DNA parts",

abstract = "Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.",

author = "Patron, {Nicola J.} and Diego Orzaez and Sylvestre Marillonnet and Heribert Warzecha and Colette Matthewman and Mark Youles and Oleg Raitskin and Aymeric Leveau and Gemma Farr{\'e} and Christian Rogers and Alison Smith and Julian Hibberd and Webb, {Alex A.R.} and James Locke and Sebastian Schornack and Jim Ajioka and Baulcombe, {David C.} and Cyril Zipfel and Sophien Kamoun and Jones, {Jonathan D.G.} and Hannah Kuhn and Silke Robatzek and {Van Esse}, {H. Peter} and Dale Sanders and Giles Oldroyd and Cathie Martin and Rob Field and Sarah O'Connor and Samantha Fox and Brande Wulff and Ben Miller and Andy Breakspear and Guru Radhakrishnan and Delaux, {Pierre Marc} and Dominique Loqu{\'e} and Antonio Granell and Alain Tissier and Patrick Shih and Brutnell, {Thomas P.} and Quick, {W. Paul} and Heiko Rischer and Fraser, {Paul D.} and Asaph Aharoni and Christine Raines and South, {Paul F.} and An{\'e}, {Jean Michel} and Hamberger, {Bj{\"o}rn R.} and Jane Langdale and Jens Stougaard and Harro Bouwmeester and Michael Udvardi and Murray, {James A.H.} and Vardis Ntoukakis and Patrick Sch{\"a}fer and Katherine Denby and Edwards, {Keith J.} and Anne Osbourn and Jim Haseloff",

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doi = "10.1111/nph.13532",

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Patron, NJ, Orzaez, D, Marillonnet, S, Warzecha, H, Matthewman, C, Youles, M, Raitskin, O, Leveau, A, Farré, G, Rogers, C, Smith, A, Hibberd, J, Webb, AAR, Locke, J, Schornack, S, Ajioka, J, Baulcombe, DC, Zipfel, C, Kamoun, S, Jones, JDG, Kuhn, H, Robatzek, S, Van Esse, HP, Sanders, D, Oldroyd, G, Martin, C, Field, R, O'Connor, S, Fox, S, Wulff, B, Miller, B, Breakspear, A, Radhakrishnan, G, Delaux, PM, Loqué, D, Granell, A, Tissier, A, Shih, P, Brutnell, TP, Quick, WP, Rischer, H, Fraser, PD, Aharoni, A, Raines, C, South, PF, Ané, JM, Hamberger, BR, Langdale, J, Stougaard, J, Bouwmeester, H, Udvardi, M, Murray, JAH, Ntoukakis, V, Schäfer, P, Denby, K, Edwards, KJ, Osbourn, A & Haseloff, J 2015, 'Standards for plant synthetic biology: A common syntax for exchange of DNA parts', New Phytologist, vol. 208, no. 1, pp. 13-19. https://doi.org/10.1111/nph.13532

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T1 - Standards for plant synthetic biology: A common syntax for exchange of DNA parts

AU - Patron, Nicola J.

AU - Orzaez, Diego

AU - Marillonnet, Sylvestre

AU - Warzecha, Heribert

AU - Matthewman, Colette

AU - Youles, Mark

AU - Raitskin, Oleg

AU - Leveau, Aymeric

AU - Farré, Gemma

AU - Rogers, Christian

AU - Smith, Alison

AU - Hibberd, Julian

AU - Webb, Alex A.R.

AU - Locke, James

AU - Schornack, Sebastian

AU - Ajioka, Jim

AU - Baulcombe, David C.

AU - Zipfel, Cyril

AU - Kamoun, Sophien

AU - Jones, Jonathan D.G.

AU - Kuhn, Hannah

AU - Robatzek, Silke

AU - Van Esse, H. Peter

AU - Sanders, Dale

AU - Oldroyd, Giles

AU - Martin, Cathie

AU - Field, Rob

AU - O'Connor, Sarah

AU - Fox, Samantha

AU - Wulff, Brande

AU - Miller, Ben

AU - Breakspear, Andy

AU - Radhakrishnan, Guru

AU - Delaux, Pierre Marc

AU - Loqué, Dominique

AU - Granell, Antonio

AU - Tissier, Alain

AU - Shih, Patrick

AU - Brutnell, Thomas P.

AU - Quick, W. Paul

AU - Rischer, Heiko

AU - Fraser, Paul D.

AU - Aharoni, Asaph

AU - Raines, Christine

AU - South, Paul F.

AU - Ané, Jean Michel

AU - Hamberger, Björn R.

AU - Langdale, Jane

AU - Stougaard, Jens

AU - Bouwmeester, Harro

AU - Udvardi, Michael

AU - Murray, James A.H.

AU - Ntoukakis, Vardis

AU - Schäfer, Patrick

AU - Denby, Katherine

AU - Edwards, Keith J.

AU - Osbourn, Anne

AU - Haseloff, Jim

N1 - Generated from Scopus record by KAUST IRTS on 2023-02-20

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

AB - Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

UR - https://onlinelibrary.wiley.com/doi/10.1111/nph.13532

UR - http://www.scopus.com/inward/record.url?scp=84939807175&partnerID=8YFLogxK

U2 - 10.1111/nph.13532

DO - 10.1111/nph.13532

M3 - Article

SN - 1469-8137

VL - 208

SP - 13

EP - 19

JO - New Phytologist

JF - New Phytologist

IS - 1

ER -

Standards for plant synthetic biology: A common syntax for exchange of DNA parts

Abstract

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ASJC Scopus subject areas

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