In situ bioprecipitation (ISBP), which involves immobilizing the metals as precipitates (mainly sulphides) in the solid phase, is an effective method of metal removal from contaminated groundwater. This study investigated the stability of metal precipitates formed after ISBP in two different solid-liquid matrices (artificial and natural). The artificial matrix consisted of sand, Zn (200mgL-1), artificial groundwater and a carbon source (electron donor). Here the stability of the Zn precipitates was evaluated by manipulation of redox and pH. The natural system matrices included aquifer material and groundwater samples collected from three different metal (Zn and Co) contaminated sites and different carbon sources were provided as electron donors. In the natural matrices, metal precipitates stability was assessed by changing aquifer redox conditions, sequential extraction, and BIOMET® assay. The results indicated that, in the artificial matrix, redox manipulation did not impact the Zn precipitates. However the sequential pH change proved detrimental, releasing 58% of the precipitated Zn back into liquid phase. In natural matrices, the applied carbon source largely affected the stability of metal precipitates. Elemental analysis performed on the precipitates formed in natural matrix showed that the main elements of the precipitates were sulphur with Zn and Co. © 2010 Elsevier B.V.