Stability and repeatability of capillary columns based on porous monoliths of poly(butyl methacrylate-co-ethylene dimethacrylate)

Laurent Geiser, Sebastiaan Eeltink, Frantisek Svec, Jean M.J. Fréchet*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

107 Scopus citations


Monolithic poly(butyl methacrylate-co-ethylene dimethacrylate) capillary columns have been prepared via either thermally or photochemically initiated polymerization of the corresponding monomers and the repeatability of their preparation has been explored. Three separate batches of 5 columns each were prepared using thermal and photochemical initiation for a total of 30 columns. All 30 capillary columns were tested in liquid chromatography-electrospray ionisation mass spectrometry mode for the separation of a model mixture of three proteins-ribonuclease A, cytochrome c and myoglobin. Excellent repeatability of retention times was observed for the proteins as evidenced by relative standard deviation (RSD) values of less than 1.5%. Somewhat broader variations with RSD values of up to 10% were observed for the pressure drop in the columns. The stability of retention times was also monitored using a single monolithic column and no significant shifts in either retention times or back pressure was observed in a series of almost 2200 consecutive protein separations.

Original languageEnglish (US)
Pages (from-to)140-146
Number of pages7
JournalJournal of Chromatography A
Issue number1-2
StatePublished - Jan 26 2007
Externally publishedYes

Bibliographical note

Funding Information:
Support of L.G. by a grant of the National Institute of General Medical Sciences, National Institutes of Health (GM44885) is gratefully acknowledged. Portions of this work were performed at the Molecular Foundry, Lawrence Berkeley National Laboratory, which is supported by the Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02—05CH11231.


  • Capillary
  • Column technology
  • Mass spectrometry
  • Monolithic columns
  • NanoHPLC
  • Proteins
  • Repeatability
  • Reproducibility
  • Validation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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