Speed breeding in growth chambers and glasshouses for crop breeding and model plant research

Sreya Ghosh; Amy Watson; Oscar E. Gonzalez-Navarro; Ricardo H. Ramirez-Gonzalez; Luis Yanes; Marcela Mendoza-Suárez; James Simmonds; Rachel Wells; Tracey Rayner; Phon Green; Amber Hafeez; Sadiye Hayta; Rachel E. Melton; Andrew Steed; Abhimanyu Sarkar; Jeremy Carter; Lionel Perkins; John Lord; Mark A. Tester; Anne Osbourn; Matthew J. Moscou; Paul Nicholson; Wendy Harwood; Cathie Martin; Claire Domoney; Cristobal Uauy; Brittany Hazard; Brande B. H. Wulff; Lee T. Hickey

doi:10.1038/s41596-018-0072-z

Speed breeding in growth chambers and glasshouses for crop breeding and model plant research

Sreya Ghosh, Amy Watson, Oscar E. Gonzalez-Navarro, Ricardo H. Ramirez-Gonzalez, Luis Yanes, Marcela Mendoza-Suárez, James Simmonds, Rachel Wells, Tracey Rayner, Phon Green, Amber Hafeez, Sadiye Hayta, Rachel E. Melton, Andrew Steed, Abhimanyu Sarkar, Jeremy Carter, Lionel Perkins, John Lord, Mark A. Tester, Anne OsbournMatthew J. Moscou, Paul Nicholson, Wendy Harwood, Cathie Martin, Claire Domoney, Cristobal Uauy, Brittany Hazard, Brande B. H. Wulff, Lee T. Hickey

Research output: Contribution to journal › Article › peer-review

286 Scopus citations

Abstract

‘Speed breeding’ (SB) shortens the breeding cycle and accelerates crop research through rapid generation advancement. SB can be carried out in numerous ways, one of which involves extending the duration of plants’ daily exposure to light, combined with early seed harvest, to cycle quickly from seed to seed, thereby reducing the generation times for some long-day (LD) or day-neutral crops. In this protocol, we present glasshouse and growth chamber–based SB approaches with supporting data from experimentation with several crops. We describe the conditions that promote the rapid growth of bread wheat, durum wheat, barley, oat, various Brassica species, chickpea, pea, grass pea, quinoa and Brachypodium distachyon. Points of flexibility within the protocols are highlighted, including how plant density can be increased to efficiently scale up plant numbers for single-seed descent (SSD). In addition, instructions are provided on how to perform SB on a small scale in a benchtop growth cabinet, enabling optimization of parameters at a low cost.

Original language	English (US)
Pages (from-to)	2944-2963
Number of pages	20
Journal	Nature Protocols
Volume	13
Issue number	12
DOIs	https://doi.org/10.1038/s41596-018-0072-z
State	Published - Nov 16 2018

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We acknowledge the support of the Biotechnology and Biological Sciences Research Council (BBSRC) strategic programmes Designing Future Wheat (BB/P016855/1), Molecules from Nature (BB/P012523/1), Understanding and Exploiting Plant and Microbial Metabolism (BB/J004561/1), Food and Health (BB/J004545/1) and Food Innovation and Health (BB/R012512/1), and also support from the Gatsby Charitable Foundation. Development of the benchtop cabinet was supported by an OpenPlant Fund grant from the joint Engineering and Physical Sciences Research Council and BBSRC-funded OpenPlant Synthetic Biology Research Centre grant BB/L014130/1. S.G. was supported by a Monsanto Beachell-Borlaug International Scholarship and the 2Blades Foundation, A.Sarkar by the BBSRC Detox Grasspea project (BB/L011719/1) and the John Innes Foundation, A.W. by an Australian Post-graduate Award and the Grains Research and Development Corporation (GRDC) Industry Top-up Scholarship (project code GRS11008), M.M.-S. by CONACYT-I2T2 Nuevo León (grant code 266954/399852), and L.T.H. by an Australian Research Council Early Career Discovery Research Award (project code DE170101296). We acknowledge M. Grantham and D. Napier from Heliospectra for their help in the choice of LED lights; L. Hernan and C. Ramírez from Newcastle University for their support and advice in the design of the benchtop cabinet; C. Moreau from the John Innes Centre and J. Ghosh from the University of Bedfordshire for help with the pea and grass pea experiments, respectively; and the JIC and UQ horticulture services for plant husbandry and their support in scaling up SB in glasshouses.

Access to Document

10.1038/s41596-018-0072-z

https://repository.kaust.edu.sa/bitstream/10754/629896/1/GhoshWatsonWulffHickey2018-bioRxiv.pdf

Cite this

Ghosh, S., Watson, A., Gonzalez-Navarro, O. E., Ramirez-Gonzalez, R. H., Yanes, L., Mendoza-Suárez, M., Simmonds, J., Wells, R., Rayner, T., Green, P., Hafeez, A., Hayta, S., Melton, R. E., Steed, A., Sarkar, A., Carter, J., Perkins, L., Lord, J., Tester, M. A., ... Hickey, L. T. (2018). Speed breeding in growth chambers and glasshouses for crop breeding and model plant research. Nature Protocols, 13(12), 2944-2963. https://doi.org/10.1038/s41596-018-0072-z

@article{942d95826ffb4a64818faa0e8ede2302,

title = "Speed breeding in growth chambers and glasshouses for crop breeding and model plant research",

abstract = "{\textquoteleft}Speed breeding{\textquoteright} (SB) shortens the breeding cycle and accelerates crop research through rapid generation advancement. SB can be carried out in numerous ways, one of which involves extending the duration of plants{\textquoteright} daily exposure to light, combined with early seed harvest, to cycle quickly from seed to seed, thereby reducing the generation times for some long-day (LD) or day-neutral crops. In this protocol, we present glasshouse and growth chamber–based SB approaches with supporting data from experimentation with several crops. We describe the conditions that promote the rapid growth of bread wheat, durum wheat, barley, oat, various Brassica species, chickpea, pea, grass pea, quinoa and Brachypodium distachyon. Points of flexibility within the protocols are highlighted, including how plant density can be increased to efficiently scale up plant numbers for single-seed descent (SSD). In addition, instructions are provided on how to perform SB on a small scale in a benchtop growth cabinet, enabling optimization of parameters at a low cost.",

author = "Sreya Ghosh and Amy Watson and Gonzalez-Navarro, {Oscar E.} and Ramirez-Gonzalez, {Ricardo H.} and Luis Yanes and Marcela Mendoza-Su{\'a}rez and James Simmonds and Rachel Wells and Tracey Rayner and Phon Green and Amber Hafeez and Sadiye Hayta and Melton, {Rachel E.} and Andrew Steed and Abhimanyu Sarkar and Jeremy Carter and Lionel Perkins and John Lord and Tester, {Mark A.} and Anne Osbourn and Moscou, {Matthew J.} and Paul Nicholson and Wendy Harwood and Cathie Martin and Claire Domoney and Cristobal Uauy and Brittany Hazard and Wulff, {Brande B. H.} and Hickey, {Lee T.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We acknowledge the support of the Biotechnology and Biological Sciences Research Council (BBSRC) strategic programmes Designing Future Wheat (BB/P016855/1), Molecules from Nature (BB/P012523/1), Understanding and Exploiting Plant and Microbial Metabolism (BB/J004561/1), Food and Health (BB/J004545/1) and Food Innovation and Health (BB/R012512/1), and also support from the Gatsby Charitable Foundation. Development of the benchtop cabinet was supported by an OpenPlant Fund grant from the joint Engineering and Physical Sciences Research Council and BBSRC-funded OpenPlant Synthetic Biology Research Centre grant BB/L014130/1. S.G. was supported by a Monsanto Beachell-Borlaug International Scholarship and the 2Blades Foundation, A.Sarkar by the BBSRC Detox Grasspea project (BB/L011719/1) and the John Innes Foundation, A.W. by an Australian Post-graduate Award and the Grains Research and Development Corporation (GRDC) Industry Top-up Scholarship (project code GRS11008), M.M.-S. by CONACYT-I2T2 Nuevo Le{\'o}n (grant code 266954/399852), and L.T.H. by an Australian Research Council Early Career Discovery Research Award (project code DE170101296). We acknowledge M. Grantham and D. Napier from Heliospectra for their help in the choice of LED lights; L. Hernan and C. Ram{\'i}rez from Newcastle University for their support and advice in the design of the benchtop cabinet; C. Moreau from the John Innes Centre and J. Ghosh from the University of Bedfordshire for help with the pea and grass pea experiments, respectively; and the JIC and UQ horticulture services for plant husbandry and their support in scaling up SB in glasshouses.",

year = "2018",

month = nov,

day = "16",

doi = "10.1038/s41596-018-0072-z",

language = "English (US)",

volume = "13",

pages = "2944--2963",

journal = "Nature Protocols",

issn = "1754-2189",

publisher = "Springer Nature",

number = "12",

}

Ghosh, S, Watson, A, Gonzalez-Navarro, OE, Ramirez-Gonzalez, RH, Yanes, L, Mendoza-Suárez, M, Simmonds, J, Wells, R, Rayner, T, Green, P, Hafeez, A, Hayta, S, Melton, RE, Steed, A, Sarkar, A, Carter, J, Perkins, L, Lord, J, Tester, MA, Osbourn, A, Moscou, MJ, Nicholson, P, Harwood, W, Martin, C, Domoney, C, Uauy, C, Hazard, B, Wulff, BBH & Hickey, LT 2018, 'Speed breeding in growth chambers and glasshouses for crop breeding and model plant research', Nature Protocols, vol. 13, no. 12, pp. 2944-2963. https://doi.org/10.1038/s41596-018-0072-z

TY - JOUR

T1 - Speed breeding in growth chambers and glasshouses for crop breeding and model plant research

AU - Ghosh, Sreya

AU - Watson, Amy

AU - Gonzalez-Navarro, Oscar E.

AU - Ramirez-Gonzalez, Ricardo H.

AU - Yanes, Luis

AU - Mendoza-Suárez, Marcela

AU - Simmonds, James

AU - Wells, Rachel

AU - Rayner, Tracey

AU - Green, Phon

AU - Hafeez, Amber

AU - Hayta, Sadiye

AU - Melton, Rachel E.

AU - Steed, Andrew

AU - Sarkar, Abhimanyu

AU - Carter, Jeremy

AU - Perkins, Lionel

AU - Lord, John

AU - Tester, Mark A.

AU - Osbourn, Anne

AU - Moscou, Matthew J.

AU - Nicholson, Paul

AU - Harwood, Wendy

AU - Martin, Cathie

AU - Domoney, Claire

AU - Uauy, Cristobal

AU - Hazard, Brittany

AU - Wulff, Brande B. H.

AU - Hickey, Lee T.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We acknowledge the support of the Biotechnology and Biological Sciences Research Council (BBSRC) strategic programmes Designing Future Wheat (BB/P016855/1), Molecules from Nature (BB/P012523/1), Understanding and Exploiting Plant and Microbial Metabolism (BB/J004561/1), Food and Health (BB/J004545/1) and Food Innovation and Health (BB/R012512/1), and also support from the Gatsby Charitable Foundation. Development of the benchtop cabinet was supported by an OpenPlant Fund grant from the joint Engineering and Physical Sciences Research Council and BBSRC-funded OpenPlant Synthetic Biology Research Centre grant BB/L014130/1. S.G. was supported by a Monsanto Beachell-Borlaug International Scholarship and the 2Blades Foundation, A.Sarkar by the BBSRC Detox Grasspea project (BB/L011719/1) and the John Innes Foundation, A.W. by an Australian Post-graduate Award and the Grains Research and Development Corporation (GRDC) Industry Top-up Scholarship (project code GRS11008), M.M.-S. by CONACYT-I2T2 Nuevo León (grant code 266954/399852), and L.T.H. by an Australian Research Council Early Career Discovery Research Award (project code DE170101296). We acknowledge M. Grantham and D. Napier from Heliospectra for their help in the choice of LED lights; L. Hernan and C. Ramírez from Newcastle University for their support and advice in the design of the benchtop cabinet; C. Moreau from the John Innes Centre and J. Ghosh from the University of Bedfordshire for help with the pea and grass pea experiments, respectively; and the JIC and UQ horticulture services for plant husbandry and their support in scaling up SB in glasshouses.

PY - 2018/11/16

Y1 - 2018/11/16

N2 - ‘Speed breeding’ (SB) shortens the breeding cycle and accelerates crop research through rapid generation advancement. SB can be carried out in numerous ways, one of which involves extending the duration of plants’ daily exposure to light, combined with early seed harvest, to cycle quickly from seed to seed, thereby reducing the generation times for some long-day (LD) or day-neutral crops. In this protocol, we present glasshouse and growth chamber–based SB approaches with supporting data from experimentation with several crops. We describe the conditions that promote the rapid growth of bread wheat, durum wheat, barley, oat, various Brassica species, chickpea, pea, grass pea, quinoa and Brachypodium distachyon. Points of flexibility within the protocols are highlighted, including how plant density can be increased to efficiently scale up plant numbers for single-seed descent (SSD). In addition, instructions are provided on how to perform SB on a small scale in a benchtop growth cabinet, enabling optimization of parameters at a low cost.

AB - ‘Speed breeding’ (SB) shortens the breeding cycle and accelerates crop research through rapid generation advancement. SB can be carried out in numerous ways, one of which involves extending the duration of plants’ daily exposure to light, combined with early seed harvest, to cycle quickly from seed to seed, thereby reducing the generation times for some long-day (LD) or day-neutral crops. In this protocol, we present glasshouse and growth chamber–based SB approaches with supporting data from experimentation with several crops. We describe the conditions that promote the rapid growth of bread wheat, durum wheat, barley, oat, various Brassica species, chickpea, pea, grass pea, quinoa and Brachypodium distachyon. Points of flexibility within the protocols are highlighted, including how plant density can be increased to efficiently scale up plant numbers for single-seed descent (SSD). In addition, instructions are provided on how to perform SB on a small scale in a benchtop growth cabinet, enabling optimization of parameters at a low cost.

UR - http://hdl.handle.net/10754/629896

UR - https://www.nature.com/articles/s41596-018-0072-z

UR - http://www.scopus.com/inward/record.url?scp=85056720446&partnerID=8YFLogxK

U2 - 10.1038/s41596-018-0072-z

DO - 10.1038/s41596-018-0072-z

M3 - Article

C2 - 30446746

SN - 1754-2189

VL - 13

SP - 2944

EP - 2963

JO - Nature Protocols

JF - Nature Protocols

IS - 12

ER -

Speed breeding in growth chambers and glasshouses for crop breeding and model plant research

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this