Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy

Benjamin Rappaz; Alexander Barbul; Annick Hoffmann; Daniel Boss; Rafi Korenstein; Christian Depeursinge; Pierre J. Magistretti; Pierre Marquet

doi:10.1016/j.bcmd.2009.01.018

Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy

Benjamin Rappaz, Alexander Barbul, Annick Hoffmann, Daniel Boss, Rafi Korenstein, Christian Depeursinge, Pierre J. Magistretti, Pierre Marquet^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9 ± 8.9 nm and 4.7 ± 0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively.

Original language	English (US)
Pages (from-to)	228-232
Number of pages	5
Journal	Blood Cells, Molecules, and Diseases
Volume	42
Issue number	3
DOIs	https://doi.org/10.1016/j.bcmd.2009.01.018
State	Published - May 2009
Externally published	Yes

Keywords

Cell membrane fluctuations
Digital holography
Quantitative phase microscopy
Red blood cell
Refractive index

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Hematology
Cell Biology

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10.1016/j.bcmd.2009.01.018

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title = "Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy",

abstract = "Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9 ± 8.9 nm and 4.7 ± 0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively.",

keywords = "Cell membrane fluctuations, Digital holography, Quantitative phase microscopy, Red blood cell, Refractive index",

author = "Benjamin Rappaz and Alexander Barbul and Annick Hoffmann and Daniel Boss and Rafi Korenstein and Christian Depeursinge and Magistretti, {Pierre J.} and Pierre Marquet",

year = "2009",

month = may,

doi = "10.1016/j.bcmd.2009.01.018",

language = "English (US)",

volume = "42",

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AU - Rappaz, Benjamin

AU - Barbul, Alexander

AU - Hoffmann, Annick

AU - Boss, Daniel

AU - Korenstein, Rafi

AU - Depeursinge, Christian

AU - Magistretti, Pierre J.

AU - Marquet, Pierre

PY - 2009/5

Y1 - 2009/5

N2 - Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9 ± 8.9 nm and 4.7 ± 0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively.

AB - Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9 ± 8.9 nm and 4.7 ± 0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively.

KW - Cell membrane fluctuations

KW - Digital holography

KW - Quantitative phase microscopy

KW - Red blood cell

KW - Refractive index

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DO - 10.1016/j.bcmd.2009.01.018

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AN - SCOPUS:64849114184

SN - 1079-9796

VL - 42

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JO - Blood Cells, Molecules, and Diseases

JF - Blood Cells, Molecules, and Diseases

IS - 3

ER -

Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy

Abstract

Keywords

ASJC Scopus subject areas

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