Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays

Adebisi Ajileye; Nataly Alvarez; Matthias Merker; Timothy M. Walker; Suriya Akter; Kerstin Brown; Danesh Moradigaravand; Thomas Schön; Sönke Andres; Viola Schleusener; Shaheed V. Omar; Francesc Coll; Hairong Huang; Roland Diel; Nazir Ismail; Julian Parkhill; Bouke C. De Jong; Tim E.A. Peto; Derrick W. Crook; Stefan Niemann; Jaime Robledo; E. Grace Smith; Sharon J. Peacock; Claudio U. Köser

doi:10.1128/AAC.02169-16

Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays

Adebisi Ajileye, Nataly Alvarez, Matthias Merker, Timothy M. Walker, Suriya Akter, Kerstin Brown, Danesh Moradigaravand, Thomas Schön, Sönke Andres, Viola Schleusener, Shaheed V. Omar, Francesc Coll, Hairong Huang, Roland Diel, Nazir Ismail, Julian Parkhill, Bouke C. De Jong, Tim E.A. Peto, Derrick W. Crook, Stefan NiemannJaime Robledo, E. Grace Smith, Sharon J. Peacock, Claudio U. Köser

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.

Original language	English (US)
Journal	Antimicrobial Agents and Chemotherapy
Volume	61
Issue number	4
DOIs	https://doi.org/10.1128/AAC.02169-16
State	Published - Apr 1 2017
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-15

ASJC Scopus subject areas

Infectious Diseases
Pharmacology
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AAC.02169-16

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Ajileye, A., Alvarez, N., Merker, M., Walker, T. M., Akter, S., Brown, K., Moradigaravand, D., Schön, T., Andres, S., Schleusener, V., Omar, S. V., Coll, F., Huang, H., Diel, R., Ismail, N., Parkhill, J., De Jong, B. C., Peto, T. E. A., Crook, D. W., ... Köser, C. U. (2017). Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays. Antimicrobial Agents and Chemotherapy, 61(4). https://doi.org/10.1128/AAC.02169-16

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title = "Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays",

abstract = "In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.",

author = "Adebisi Ajileye and Nataly Alvarez and Matthias Merker and Walker, {Timothy M.} and Suriya Akter and Kerstin Brown and Danesh Moradigaravand and Thomas Sch{\"o}n and S{\"o}nke Andres and Viola Schleusener and Omar, {Shaheed V.} and Francesc Coll and Hairong Huang and Roland Diel and Nazir Ismail and Julian Parkhill and {De Jong}, {Bouke C.} and Peto, {Tim E.A.} and Crook, {Derrick W.} and Stefan Niemann and Jaime Robledo and Smith, {E. Grace} and Peacock, {Sharon J.} and K{\"o}ser, {Claudio U.}",

note = "Generated from Scopus record by KAUST IRTS on 2023-02-15",

year = "2017",

month = apr,

day = "1",

doi = "10.1128/AAC.02169-16",

language = "English (US)",

volume = "61",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "1098-6596",

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Ajileye, A, Alvarez, N, Merker, M, Walker, TM, Akter, S, Brown, K, Moradigaravand, D, Schön, T, Andres, S, Schleusener, V, Omar, SV, Coll, F, Huang, H, Diel, R, Ismail, N, Parkhill, J, De Jong, BC, Peto, TEA, Crook, DW, Niemann, S, Robledo, J, Smith, EG, Peacock, SJ & Köser, CU 2017, 'Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays', Antimicrobial Agents and Chemotherapy, vol. 61, no. 4. https://doi.org/10.1128/AAC.02169-16

Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays. / Ajileye, Adebisi; Alvarez, Nataly; Merker, Matthias et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 61, No. 4, 01.04.2017.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays

AU - Ajileye, Adebisi

AU - Alvarez, Nataly

AU - Merker, Matthias

AU - Walker, Timothy M.

AU - Akter, Suriya

AU - Brown, Kerstin

AU - Moradigaravand, Danesh

AU - Schön, Thomas

AU - Andres, Sönke

AU - Schleusener, Viola

AU - Omar, Shaheed V.

AU - Coll, Francesc

AU - Huang, Hairong

AU - Diel, Roland

AU - Ismail, Nazir

AU - Parkhill, Julian

AU - De Jong, Bouke C.

AU - Peto, Tim E.A.

AU - Crook, Derrick W.

AU - Niemann, Stefan

AU - Robledo, Jaime

AU - Smith, E. Grace

AU - Peacock, Sharon J.

AU - Köser, Claudio U.

N1 - Generated from Scopus record by KAUST IRTS on 2023-02-15

PY - 2017/4/1

Y1 - 2017/4/1

N2 - In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.

AB - In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.

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U2 - 10.1128/AAC.02169-16

DO - 10.1128/AAC.02169-16

M3 - Article

SN - 1098-6596

VL - 61

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 4

ER -

Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

Access to Document

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