SMYD2 is induced during cell differentiation and participates in early development

Borja Sesé; Maria J. Barrero; Maria Carme Fabregat; Veronika Sander; Juan Carlos Izpisua-Belmonte

doi:10.1387/ijdb.130051ji

SMYD2 is induced during cell differentiation and participates in early development

Borja Sesé, Maria J. Barrero, Maria Carme Fabregat, Veronika Sander, Juan Carlos Izpisua-Belmonte

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Histone modifying enzymes play critical roles in cell differentiation and development. In this study, we report that SMYD2 (SET and MYND domain containing protein 2), a histone lysine methyltransferase, is induced during human embryonic stem (ES) cell differentiation and it is preferentially expressed in somatic cells versus pluripotent cells. Knockdown of SMYD2 in human ES cells promotes the induction of endodermal markers during differentiation, while overexpression has opposite effects. In vivo experiments in zebrafish revealed that knockdown of smyd2a (a homologue gene of human SMYD2) causes developmental delay and aberrant tail formation, which is coincident with low expression of ntl and over induction Nodal-related genes during gastrulation. Taken together, these findings suggest that SMYD2 plays a critical role at early stages of development and in human ES cell differentiation.

Original language	English (US)
Pages (from-to)	357-364
Number of pages	8
Journal	International Journal of Developmental Biology
Volume	57
Issue number	5
DOIs	https://doi.org/10.1387/ijdb.130051ji
State	Published - 2013
Externally published	Yes

Keywords

Development
Differentiation
Methyltransferase
SMYD2
Stem cell

ASJC Scopus subject areas

Embryology
Developmental Biology

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10.1387/ijdb.130051ji

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title = "SMYD2 is induced during cell differentiation and participates in early development",

abstract = "Histone modifying enzymes play critical roles in cell differentiation and development. In this study, we report that SMYD2 (SET and MYND domain containing protein 2), a histone lysine methyltransferase, is induced during human embryonic stem (ES) cell differentiation and it is preferentially expressed in somatic cells versus pluripotent cells. Knockdown of SMYD2 in human ES cells promotes the induction of endodermal markers during differentiation, while overexpression has opposite effects. In vivo experiments in zebrafish revealed that knockdown of smyd2a (a homologue gene of human SMYD2) causes developmental delay and aberrant tail formation, which is coincident with low expression of ntl and over induction Nodal-related genes during gastrulation. Taken together, these findings suggest that SMYD2 plays a critical role at early stages of development and in human ES cell differentiation.",

keywords = "Development, Differentiation, Methyltransferase, SMYD2, Stem cell",

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year = "2013",

doi = "10.1387/ijdb.130051ji",

language = "English (US)",

volume = "57",

pages = "357--364",

journal = "International Journal of Developmental Biology",

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publisher = "UPV/EHU Press",

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T1 - SMYD2 is induced during cell differentiation and participates in early development

AU - Sesé, Borja

AU - Barrero, Maria J.

AU - Fabregat, Maria Carme

AU - Sander, Veronika

AU - Izpisua-Belmonte, Juan Carlos

PY - 2013

Y1 - 2013

N2 - Histone modifying enzymes play critical roles in cell differentiation and development. In this study, we report that SMYD2 (SET and MYND domain containing protein 2), a histone lysine methyltransferase, is induced during human embryonic stem (ES) cell differentiation and it is preferentially expressed in somatic cells versus pluripotent cells. Knockdown of SMYD2 in human ES cells promotes the induction of endodermal markers during differentiation, while overexpression has opposite effects. In vivo experiments in zebrafish revealed that knockdown of smyd2a (a homologue gene of human SMYD2) causes developmental delay and aberrant tail formation, which is coincident with low expression of ntl and over induction Nodal-related genes during gastrulation. Taken together, these findings suggest that SMYD2 plays a critical role at early stages of development and in human ES cell differentiation.

AB - Histone modifying enzymes play critical roles in cell differentiation and development. In this study, we report that SMYD2 (SET and MYND domain containing protein 2), a histone lysine methyltransferase, is induced during human embryonic stem (ES) cell differentiation and it is preferentially expressed in somatic cells versus pluripotent cells. Knockdown of SMYD2 in human ES cells promotes the induction of endodermal markers during differentiation, while overexpression has opposite effects. In vivo experiments in zebrafish revealed that knockdown of smyd2a (a homologue gene of human SMYD2) causes developmental delay and aberrant tail formation, which is coincident with low expression of ntl and over induction Nodal-related genes during gastrulation. Taken together, these findings suggest that SMYD2 plays a critical role at early stages of development and in human ES cell differentiation.

KW - Development

KW - Differentiation

KW - Methyltransferase

KW - SMYD2

KW - Stem cell

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SP - 357

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JO - International Journal of Developmental Biology

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ER -

SMYD2 is induced during cell differentiation and participates in early development

Abstract

Keywords

ASJC Scopus subject areas

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