Single strand conformation polymorphism analysis of PCR-tDNA fingerprinting to address the identification of Bacillus species

Sara Borin; Daniele Daffonchio; Claudia Sorlini

doi:10.1016/S0378-1097(97)00458-8

Single strand conformation polymorphism analysis of PCR-tDNA fingerprinting to address the identification of Bacillus species

Sara Borin, Daniele Daffonchio^*, Claudia Sorlini

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The suitability of tDNA-PCR fingerprinting to identify species of the genus Bacillus was tested on 75 strains. Strains belonging to the same species or the same phylogenetic cluster were correctly grouped. Among B. stearothermophilus strains, different pattern types were found. This could be due to the unclear taxonomic situation of these strains, rather than to a failure of the tDNA-PCR. Single strand conformation polymorphism (SSCP) of the PCR products allowed species discrimination within the 'B. subtilis group', but not within the 'B. cereus group'. The tDNA-PCR, alone or coupled with SSCP analysis, is useful to address Bacillus species identification, particularly for those species which are not phylogenetically tightly clustered.

Original language	English (US)
Pages (from-to)	87-93
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	157
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(97)00458-8
State	Published - Dec 1 1997
Externally published	Yes

Keywords

Bacillus
Bacillus cereus
Bacillus subtilis
Identification
SSCP
tDNA fingerprinting

ASJC Scopus subject areas

Genetics
Molecular Biology
Microbiology

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10.1016/S0378-1097(97)00458-8

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title = "Single strand conformation polymorphism analysis of PCR-tDNA fingerprinting to address the identification of Bacillus species",

abstract = "The suitability of tDNA-PCR fingerprinting to identify species of the genus Bacillus was tested on 75 strains. Strains belonging to the same species or the same phylogenetic cluster were correctly grouped. Among B. stearothermophilus strains, different pattern types were found. This could be due to the unclear taxonomic situation of these strains, rather than to a failure of the tDNA-PCR. Single strand conformation polymorphism (SSCP) of the PCR products allowed species discrimination within the 'B. subtilis group', but not within the 'B. cereus group'. The tDNA-PCR, alone or coupled with SSCP analysis, is useful to address Bacillus species identification, particularly for those species which are not phylogenetically tightly clustered.",

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author = "Sara Borin and Daniele Daffonchio and Claudia Sorlini",

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doi = "10.1016/S0378-1097(97)00458-8",

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T1 - Single strand conformation polymorphism analysis of PCR-tDNA fingerprinting to address the identification of Bacillus species

AU - Borin, Sara

AU - Daffonchio, Daniele

AU - Sorlini, Claudia

PY - 1997/12/1

Y1 - 1997/12/1

N2 - The suitability of tDNA-PCR fingerprinting to identify species of the genus Bacillus was tested on 75 strains. Strains belonging to the same species or the same phylogenetic cluster were correctly grouped. Among B. stearothermophilus strains, different pattern types were found. This could be due to the unclear taxonomic situation of these strains, rather than to a failure of the tDNA-PCR. Single strand conformation polymorphism (SSCP) of the PCR products allowed species discrimination within the 'B. subtilis group', but not within the 'B. cereus group'. The tDNA-PCR, alone or coupled with SSCP analysis, is useful to address Bacillus species identification, particularly for those species which are not phylogenetically tightly clustered.

AB - The suitability of tDNA-PCR fingerprinting to identify species of the genus Bacillus was tested on 75 strains. Strains belonging to the same species or the same phylogenetic cluster were correctly grouped. Among B. stearothermophilus strains, different pattern types were found. This could be due to the unclear taxonomic situation of these strains, rather than to a failure of the tDNA-PCR. Single strand conformation polymorphism (SSCP) of the PCR products allowed species discrimination within the 'B. subtilis group', but not within the 'B. cereus group'. The tDNA-PCR, alone or coupled with SSCP analysis, is useful to address Bacillus species identification, particularly for those species which are not phylogenetically tightly clustered.

KW - Bacillus

KW - Bacillus cereus

KW - Bacillus subtilis

KW - Identification

KW - SSCP

KW - tDNA fingerprinting

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Single strand conformation polymorphism analysis of PCR-tDNA fingerprinting to address the identification of Bacillus species

Abstract

Keywords

ASJC Scopus subject areas

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