Single-shot, simultaneous incoherent and holographic microscopy

E. Shaffer*, N. Pavillon, Christian Depeursinge

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


In this work, we demonstrate single-shot, simultaneous recording and subsequent retrieval of one incoherent and two holographic (intensity and phase) images from the same camera frame. Demultiplexing of incoherent and holographic signals in the spatial frequency domain is made possible by carrier frequency modulation and spatial oversampling intrinsic to the off-axis digital holographic configuration. In particular, we show applications to combined fluorescence and digital holographic microscopy, as well as combined bright field and holographic second harmonic generation microscopy.

Original languageEnglish (US)
Pages (from-to)49-62
Number of pages14
JournalJournal of Microscopy
Issue number1
StatePublished - Jan 1 2012


  • Bright field
  • Digital holography
  • Fluorescence
  • Holographic multiplexing
  • Phase imaging
  • Second harmonic generation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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