Single-molecule surface enhanced resonance Raman spectroscopy of the enhanced green fluorescent protein

Satoshi Habuchi; Mircea Cotlet; Roel Gronheid; Gunter Dirix; Jan Michiels; Jos Vanderleyden; Frans C. De Schryver; Johan Hofkens

doi:10.1021/ja0353311

Single-molecule surface enhanced resonance Raman spectroscopy of the enhanced green fluorescent protein

Satoshi Habuchi, Mircea Cotlet, Roel Gronheid, Gunter Dirix, Jan Michiels, Jos Vanderleyden, Frans C. De Schryver, Johan Hofkens^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

152 Scopus citations

Abstract

In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level.

Original language	English (US)
Pages (from-to)	8446-8447
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	125
Issue number	28
DOIs	https://doi.org/10.1021/ja0353311
State	Published - Jul 16 2003
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
Biochemistry
Catalysis
Colloid and Surface Chemistry

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10.1021/ja0353311

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abstract = "In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level.",

author = "Satoshi Habuchi and Mircea Cotlet and Roel Gronheid and Gunter Dirix and Jan Michiels and Jos Vanderleyden and {De Schryver}, {Frans C.} and Johan Hofkens",

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AU - Habuchi, Satoshi

AU - Cotlet, Mircea

AU - Gronheid, Roel

AU - Dirix, Gunter

AU - Michiels, Jan

AU - Vanderleyden, Jos

AU - De Schryver, Frans C.

AU - Hofkens, Johan

PY - 2003/7/16

Y1 - 2003/7/16

N2 - In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level.

AB - In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level.

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 28

ER -

Single-molecule surface enhanced resonance Raman spectroscopy of the enhanced green fluorescent protein

Abstract

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