Abstract
This paper presents a 40 GHz microwave biosensor used to monitor and characterize single cells (THP-1) subjected to electrochemotherapy and obtain an electronic signature of the treatment efficiency. This biosensor proposes a non-destructive and label-free technique that first allows, with the rapid measurement of single untreated cells in their culture medium, the extraction of two frequency-dependent dielectric parameters, the capacitance (C (f)) and the conductance (G (f)). Second, this technique can powerfully reveal the effects of a chemical membrane permeabilizing treatment (Saponin). At last, it permits us to detect, and predict, the potentiation of a molecule classically used in chemotherapy (Bleomycin) when combined with the application of electric pulses (principle of electrochemotherapy). Treatment-affected cells show a decrease in the capacitive and conductive contrasts, indicating damages at the cellular levels. Along with these results, classical biological tests are conducted. Statistical analysis points out a high correlation rate (R2>0.97), which clearly reveals the reliability and efficacy of our technique and makes it an attractive technique for biology related researches and personalized medicine.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-1 |
| Number of pages | 1 |
| Journal | IEEE Transactions on Biomedical Engineering |
| DOIs | |
| State | Published - Apr 26 2022 |
| Externally published | Yes |
Bibliographical note
KAUST Repository Item: Exported on 2022-10-27Acknowledgements: This work was supported in part by Toulouse COMUE and Region Midi-Pyrénées, France, and in part by LAAS-CNRS micro and nano technologies platform, a member of the RENATECH French National Network.