Single Cell Microwave Biosensor for Monitoring Cellular Response to Electrochemotherapy

Amar Tamra, Amel Zedek, Marie-Pierre Rols, David Dubuc, Katia Grenier

Research output: Contribution to journalArticlepeer-review

Abstract

This paper presents a 40 GHz microwave biosensor used to monitor and characterize single cells (THP-1) subjected to electrochemotherapy and obtain an electronic signature of the treatment efficiency. This biosensor proposes a non-destructive and label-free technique that first allows, with the rapid measurement of single untreated cells in their culture medium, the extraction of two frequency-dependent dielectric parameters, the capacitance (C (f)) and the conductance (G (f)). Second, this technique can powerfully reveal the effects of a chemical membrane permeabilizing treatment (Saponin). At last, it permits us to detect, and predict, the potentiation of a molecule classically used in chemotherapy (Bleomycin) when combined with the application of electric pulses (principle of electrochemotherapy). Treatment-affected cells show a decrease in the capacitive and conductive contrasts, indicating damages at the cellular levels. Along with these results, classical biological tests are conducted. Statistical analysis points out a high correlation rate (R2>0.97), which clearly reveals the reliability and efficacy of our technique and makes it an attractive technique for biology related researches and personalized medicine.
Original languageEnglish (US)
Pages (from-to)1-1
Number of pages1
JournalIEEE Transactions on Biomedical Engineering
DOIs
StatePublished - Apr 26 2022
Externally publishedYes

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