TY - JOUR
T1 - Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1
AU - Guan, Yue
AU - Haroon, Mohamed
AU - Alam, Intikhab
AU - Ferry, James G.
AU - Stingl, Ulrich
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This study was supported by King Abdullah University of Science and Technology (KAUST) through baseline funding and the SEDCO Research Excellence award to US.
PY - 2017/6/6
Y1 - 2017/6/6
N2 - Pyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functionality of methylamine methyltransferases, an important component of corrinoid-dependent methyltransfer reactions, depends on the presence of Pyl. Here, we present a putative pyl gene cluster obtained from single-cell genomes of the archaeal Mediterranean Sea Brine Lakes group 1 (MSBL1) from the Red Sea. Functional annotation of the MSBL1 single cell amplified genomes (SAGs) also revealed a complete corrinoid-dependent methyl-transfer pathway suggesting that members of MSBL1 may possibly be capable of synthesizing Pyl and metabolizing methylated amines. This article is protected by copyright. All rights reserved.
AB - Pyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functionality of methylamine methyltransferases, an important component of corrinoid-dependent methyltransfer reactions, depends on the presence of Pyl. Here, we present a putative pyl gene cluster obtained from single-cell genomes of the archaeal Mediterranean Sea Brine Lakes group 1 (MSBL1) from the Red Sea. Functional annotation of the MSBL1 single cell amplified genomes (SAGs) also revealed a complete corrinoid-dependent methyl-transfer pathway suggesting that members of MSBL1 may possibly be capable of synthesizing Pyl and metabolizing methylated amines. This article is protected by copyright. All rights reserved.
UR - http://hdl.handle.net/10754/623647
UR - http://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12545/abstract
UR - http://www.scopus.com/inward/record.url?scp=85020165438&partnerID=8YFLogxK
U2 - 10.1111/1758-2229.12545
DO - 10.1111/1758-2229.12545
M3 - Article
C2 - 28493460
SN - 1758-2229
VL - 9
SP - 404
EP - 410
JO - Environmental Microbiology Reports
JF - Environmental Microbiology Reports
IS - 4
ER -