Single amino acid change alters specificity of the multi-allelic wheat stem rust resistance locus SR9

Jianping Zhang, Jayaveeramuthu Nirmala, Shisheng Chen, Matthias Jost, Burkhard Steuernagel, Mirka Karafiatova, Tim Hewitt, Hongna Li, Erena Edae, Keshav Sharma, Sami Hoxha, Dhara Bhatt, Rea Antoniou-Kourounioti, Peter Dodds, Brande B.H. Wulff, Jaroslav Dolezel, Michael Ayliffe, Colin Hiebert, Robert McIntosh, Jorge DubcovskyPeng Zhang*, Matthew N. Rouse*, Evans Lagudah*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Most rust resistance genes thus far isolated from wheat have a very limited number of functional alleles. Here, we report the isolation of most of the alleles at wheat stem rust resistance gene locus SR9. The seven previously reported resistance alleles (Sr9a, Sr9b, Sr9d, Sr9e, Sr9f, Sr9g, and Sr9h) are characterised using a synergistic strategy. Loss-of-function mutants and/or transgenic complementation are used to confirm Sr9b, two haplotypes of Sr9e (Sr9e_h1 and Sr9e_h2), Sr9g, and Sr9h. Each allele encodes a highly related nucleotide-binding site leucine-rich repeat (NB-LRR) type immune receptor, containing an unusual long LRR domain, that confers resistance to a unique spectrum of isolates of the wheat stem rust pathogen. The only SR9 protein effective against stem rust pathogen race TTKSK (Ug99), SR9H, differs from SR9B by a single amino acid. SR9B and SR9G resistance proteins are also distinguished by only a single amino acid. The SR9 allelic series found in the B subgenome are orthologs of wheat stem rust resistance gene Sr21 located in the A subgenome with around 85% identity in protein sequences. Together, our results show that functional diversification of allelic variants at the SR9 locus involves single and multiple amino acid changes that recognize isolates of wheat stem rust.

Original languageEnglish (US)
Article number7354
JournalNature Communications
Volume14
Issue number1
DOIs
StatePublished - Dec 2023

Bibliographical note

Publisher Copyright:
© 2023, This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry, Genetics and Molecular Biology
  • General Physics and Astronomy

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