Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium

Benjamin Rappaz, Florian Charrière, Christian Depeursinge, Pierre J. Magistretti, Pierre Marquet*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

173 Scopus citations


Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelength DHM that allows us to separately measure both the RI and the cellular thickness by exploiting an enhanced dispersion of the perfusion medium achieved by the utilization of an extracellular dye. The two wavelengths are chosen in the vicinity of the absorption peak of the dye, where the absorption is accompanied by a significant variation of the RI as a function of the wavelength.

Original languageEnglish (US)
Pages (from-to)744-746
Number of pages3
JournalOptics Letters
Issue number7
StatePublished - Apr 1 2008
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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