Simple and effective graphene laser processing for neuron patterning application

Matteo Lorenzoni*, Fernando Brandi, Silvia Dante, Andrea Giugni, Bruno Torre

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


A straightforward fabrication technique to obtain patterned substrates promoting ordered neuron growth is presented. Chemical vapor deposition (CVD) single layer graphene (SLG) was machined by means of single pulse UV laser ablation technique at the lowest effective laser fluence in order to minimize laser damage effects. Patterned substrates were then coated with poly-D-lysine by means of a simple immersion in solution. Primary embryonic hippocampal neurons were cultured on our substrate, demonstrating an ordered interconnected neuron pattern mimicking the pattern design. Surprisingly, the functionalization is more effective on the SLG, resulting in notably higher alignment for neuron adhesion and growth. Therefore the proposed technique should be considered a valuable candidate to realize a new generation of highly specialized biosensors.

Original languageEnglish (US)
Article number1954
JournalScientific Reports
StatePublished - 2013

ASJC Scopus subject areas

  • General


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